Phytochemical Investigation and Biological Activity of Doronicum Pardalianches L. Roots Against Alzheimer’S Disease Publisher Pubmed



Manayi A¹ ; Rastegari A² ; Heydarian B³ ; Vahedimazdabadi Y² ; Khanavi M⁴ ; Akbarzadeh T^{2, 3} ; Saeedi M^{1, 2} Show Affiliations
Source: Natural Product Research Published:2023

Abstract

The methanol extract of Doronicum pardalianches L. was fractionated using n-hexane, chloroform, and ethyl acetate to evaluate their cholinesterase (ChE) inhibitory activity via modified Ellman’s method. It was perceived that only the ethyl acetate fraction was active toward acetylcholinesterase (AChE) with IC50 value of 172.21 µg/mL. Also, all fractions showed no butyrylcholinesterase (BChE) inhibitory activity. The ethyl acetate fraction was also investigated for its neuroprotectivity and metal chelating ability (Zn2+, Fe2+, and Cu2+) which demonstrated desired activity. Phytochemical analysis of the ethyl acetate fraction led to isolation and identification of formononetin 7-O-β-D-glucopyranoside which has not been previously reported for this plant. © 2021 Informa UK Limited, trading as Taylor & Francis Group.