Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients

Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients Publisher Pubmed

Voshagh Q¹ ; Anoshiravani A² ; Karimpour A¹ ; Goodarzi G³ ; Tehrani SS⁴ ; Tabatabaeimalazy O^{5, 6} ; Panahi G¹

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1. Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Digestive Disease Research Center, Digestive Disease Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pathobiology and Laboratory Sciences, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran
4. Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Science, Tehran, Iran
5. Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
6. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Immunity# Inflammation and Disease Published:2024

Abstract

Background: Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by numerous factors, such as immune system dysfunction and genetic factors. MicroRNAs (miRNAs) play a crucial role in UC pathogenesis, particularly via the JAK-STAT pathway. Our aim was to investigate the association between miRNA-101 and JAK2-STAT3 signaling pathway with inflammatory cytokines in UC patients. Methods: We enrolled 35 UC patients and 35 healthy individuals as the control group, referred to Shariati Hospital, Tehran, Iran. Patients were diagnosed based on clinical, laboratory, histological, and colonoscopy criteria. RNA and protein extracted from tissue samples. Real-time PCR was used to assess the expression levels of miRNA-101, interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and IL-10 genes, while western blot was employed to measure levels of P-STAT3, total STAT3, and JAK2 proteins. Results: Expression of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 significantly increased, while the expression of IL-10 significantly decreased in the case group versus controls. Additionally, miRNA-101 expression was significantly higher in UC patients. A significant correlation between miRNA-101 and IL-6 expression was observed, indicating their relationship and possible impact on cell signaling pathways, JAK2-STAT3. No significant changes were observed in phosphorylated and total STAT3 and JAK2 protein expression. Conclusion: This study provides evidence of increased miRNA-101 expression in UC tissue, suggesting a potential correlation between miRNA-101 and IL-6 expression and their involvement in the JAK2-STAT3 pathway. The study confirms alterations in UC patients' pro-inflammatory cytokines and anti-inflammatory IL-10. However, further investigations are needed to understand the exact role of miRNA-101 in UC pathogenesis fully. © 2024 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

2. Expression Analysis of Long Noncoding Rna-Malat1 and Interleukin-6 in Inflammatory Bowel Disease Patients, Iranian Journal of Allergy# Asthma and Immunology (2023)

3. Decreased Serum Level of Mir-155 Is Associated With Obesity and Its Related Metabolic Traits, Clinical Laboratory (2018)

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Farideh Razi (1)

Ghodratollah Panahi (1)

Style	Citing Format
MLA	Voshagh Q, et al.. "Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients." Immunity# Inflammation and Disease, vol. 12, no. 3, 2024, pp. -.
APA	Voshagh Q, Anoshiravani A, Karimpour A, Goodarzi G, Tehrani SS, Tabatabaeimalazy O, Panahi G (2024). Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients. Immunity# Inflammation and Disease, 12(3), -.
Chicago	Voshagh Q, Anoshiravani A, Karimpour A, Goodarzi G, Tehrani SS, Tabatabaeimalazy O, Panahi G. "Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients." Immunity# Inflammation and Disease 12, no. 3 (2024): -.
Harvard	Voshagh Q et al. (2024) 'Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients', Immunity# Inflammation and Disease, 12(3), pp. -.
Vancouver	Voshagh Q, Anoshiravani A, Karimpour A, Goodarzi G, Tehrani SS, Tabatabaeimalazy O, et al.. Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients. Immunity# Inflammation and Disease. 2024;12(3):-.
BibTex	@article{ author = {Voshagh Q and Anoshiravani A and Karimpour A and Goodarzi G and Tehrani SS and Tabatabaeimalazy O and Panahi G}, title = {Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients}, journal = {Immunity# Inflammation and Disease}, volume = {12}, number = {3}, pages = {-}, year = {2024} }
RIS	TY - JOUR AU - Voshagh Q AU - Anoshiravani A AU - Karimpour A AU - Goodarzi G AU - Tehrani SS AU - Tabatabaeimalazy O AU - Panahi G TI - Investigating the Association Between the Tissue Expression of Mirna-101, Jak2/Stat3 With Tnf-Α, Il-6, Il-1Β, and Il-10 Cytokines in the Ulcerative Colitis Patients JO - Immunity# Inflammation and Disease VL - 12 IS - 3 SP - EP - PY - 2024 ER -

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