Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Down-Expression of Mirna-98 and Over-Expression of Mirna-9 Can Result in Inadequate Immune System Response Against Lung Cancer Publisher



Farzanegan B1, 11 ; Bahrami N2, 12 ; Birjandi B3 ; Khosravi A4 ; Nasab AF5 ; Fathi M3 ; Dizaj MKP6 ; Daustani M7 ; Askari E8 ; Mohamadnia A8, 9 ; Velayati AA10
Authors
Show Affiliations
Authors Affiliations
  1. 1. Critical Care Quality Improvement Research Center at Shahid Modarres Hospital, Department of Anaesthesiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Anesthesiology Research Center, Anesthesia and Critical Care Department, Shahid Modares Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Tobacco Prevention and Control Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Behavioral Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Biostatistics Department, Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  7. 7. Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran
  8. 8. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  9. 9. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  10. 10. Mycobacteriology Research Centre (MRC), National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  11. 11. Tracheal Diseases research center, National Research Institute of Tuberculosis and Lung Diseases (NRITlD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  12. 12. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

Source: Biointerface Research in Applied Chemistry Published:2021


Abstract

Lung cancer is a disease presenting high incidence and mortality rates. Its most common type is non-small cell lung cancer (NSCLC), constituting about 80-85% of all cases. MicroRNAs (miRNAs) are the molecules playing a role in the regulation of genes. Two crucial cytokines in cancer are interleukin (IL)-10 and IFN-γ. Our objective was to investigate the expression of miRNA-9, miRNA-98, JAK, and STAT3 in patients with NSCLC and detect the cytokine level of IL-10. A total of 50 patients with NSCLC participated in this study. Besides, 50 healthy blood samples were selected as the control group. Real-time PCR determined the expression levels of miRNAs so that the RNAs extracted from the patients' peripheral mononuclear cells (PBMC) were initially synthesized, and cDNA was then extracted. Finally, the synthesized cDNA was amplified using real-time PCR, and its expression was compared to the control group. ELISA technique was used to detect IL-10 in plasma. Our result showed a low level of expression of miRNA-98, JAK, and STAT3 and a high-level expression of miRNA-9. ELISA test indicated a high cytokine level of IL-10 in the NSCLC patients' serum compared to the healthy controls. MiRNA-9 could suppress JAK and STAT3 genes in the NSCLC patients, which causes the poor immune response against the cell's proliferation. Besides, IL-10 was enhanced when miRNA-98 was reduced. Therefore, the elevation of IL-10, as an anti-inflammatory cytokine, could lead to lung cancer progression without a strong immune system response. © 2021 by the authors.
Related Docs
1. The Impact of Mir-9 Regulation in Normal and Malignant Hematopoiesis, Oncology Reviews (2018)
2. Molecular Identification of Mir-145 and Mir-9 Expression Level As Prognostic Biomarkers for Early-Stage Cervical Cancer Detection, QJM: An International Journal of Medicine (2017)
3. Emerging Molecular Functions of Microrna-9: Cancer Pathology and Therapeutic Implications, Anti-Cancer Agents in Medicinal Chemistry (2021)
4. Regulation of Dna Methylation Machinery by Epi-Mirnas in Human Cancer: Emerging New Targets in Cancer Therapy, Cancer Gene Therapy (2021)
5. Reciprocal Interconnection of Mirnome-Epigenome in Cancer Pathogenesis and Its Therapeutic Potential, Epigenetics Territory and Cancer (2015)
6. Cancer Genetics at a Glance: The Comprehensive Insights, Cancer Genetics and Psychotherapy (2017)
7. Inflammation Related Mirnas As an Important Player Between Obesity and Cancers, Journal of Diabetes and Metabolic Disorders (2019)
8. Micrornas As Biomarkers for Early Diagnosis, Targeting and Prognosis of Prostate Cancer, Pathology Research and Practice (2023)
Experts (# of related papers)
View all Related Experts
Shirin Hasani-Ranjbar (1)
Shaban Alizadeh (1)