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Molecular Identification of Some Haemaphysalis Species (Acari: Ixodidae) Using Mitochondrial and Nuclear Evidences in Parts of Iran Publisher



Faghihi F1, 2 ; Hosseinichegeni A3 ; Edalat H4 ; Banafshi O5 ; Telmadarraiy Z4 ; Sedaghat MM4
Authors
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Authors Affiliations
  1. 1. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  3. 3. Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran
  4. 4. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Disease Control, Deputy of Health, Kurdistan University of Medical Sciences, Sanandaj, Iran

Source: Systematic and Applied Acarology Published:2020


Abstract

Haemaphysalis is the second largest ixodid tick genus. We decided to identify the Iranian Haemaphysalis species with the analysis of mitochondrial (COI & 16S rRNA) and nuclear (ITS2) gene sequences. Finally, 21 PCR products were sequenced, successfully. Phylogenetic trees were analyzed using BEAST software and Bayesian Inference method. Ha. sulcata and Ha. punctata were well differentiated using the 16S rRNA, COI and ITS2 genes. According to 16S rRNA and COI phylogenetic trees Ha. inermis is situated as an ancestral clade. © Systematic & Applied Acarology Society.