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Molecular Detection of Theileria Ovis (Apicomplexa: Theileriidae), Anaplasma Ovis (Rickettsiales: Anaplasmataceae), and Mycoplasma Sp. (Tenericutes: Mycoplasmataceae) From Sheep Blood in Western Iran Publisher



Goudarzi G1 ; Tavakoli M2 ; Ezatpour B3 ; Kooshki H4 ; Hosseinichegeni A3, 5
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
  2. 2. Agricultural Research, Education and Extension Organization (AREEO), Lorestan Agricultural & Natural Resources Research Center, Khorramabad, Iran
  3. 3. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
  4. 4. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Pol-e Dokhtar Higher Education Center, University of Lorestan, Pol-e Dokhtar, Iran

Source: Comparative Clinical Pathology Published:2019


Abstract

The present study was designed to detect tick-borne hemoparasitic agents in 35 blood samples collected from healthy sheep from Lorestan province located in western Iran. Sheep blood samples were examined only using molecular method. By polymerase chain reaction (PCR), 13 (65%), 3 (15%), and one samples (5%) were to be infected by Anaplasma ovis, Theileria ovis, and Mycoplasma species, respectively. One sample from each positive target PCR was sequenced, successfully. This study is an investigation on tick-borne pathogens of domestic sheep, and a Mycoplasma agent is reported for the first time in Iran. © 2019, Springer-Verlag London Ltd., part of Springer Nature.