In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects

In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects Publisher

Salehi M^{1, 2} ; Ai A³ ; Ehterami A⁴ ; Einabadi M⁵ ; Taslimi A⁶ ; Ai A³ ; Akbarzadeh H⁶ ; Ameli GJ⁶ ; Farzamfar S⁷ ; Shirian S⁸ ; Azimi N⁶ ; Sadeghi F⁶ ; Bahrami N^{9, 10} ; Goodarzi A¹¹ Show All Authors

Salehi M^{1, 2}
Ai A³
Ehterami A⁴
Einabadi M⁵
Taslimi A⁶
Ai A³
Akbarzadeh H⁶
Ameli GJ⁶
Farzamfar S⁷
Shirian S⁸
Azimi N⁶
Sadeghi F⁶
Bahrami N^{9, 10}
Goodarzi A¹¹
Ai J⁷

Show Affiliations

1. Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
2. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran
3. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Mechanical and Aerospace Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran
5. Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
6. Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
7. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran
8. Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
9. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
10. Department of Oral and Maxillofacial Surgery, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
11. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran

Source: Nanomedicine Journal Published:2020

Abstract

Objective(s): Bone tissue engineering is aimed at the fabrication of bone graft to ameliorate bone defects without using autografts or allografts. Materials and Methods: In the present study, the coprecipitation method was used to prepare hydroxyapatite (HA) nanoparticles containing nandrolone. To do so, 12.5, 25, and 50 mg of nandrolone were loaded into poly(lactic acid) (PLA)/nano-HA, and the freeze casting method was used to fabricate porous scaffolds. The morphology, mechanical strength, wettability, porosity, degradation, blood compatibility, and cellular response of the scaffolds were evaluated using various tests. For further investigation, the developed scaffolds were incorporated into the rat calvaria defect model, and their effects on bone healing were evaluated. Results: The obtained results indicated that the fabricated scaffolds had the approximate porosity of 80% and compress strength of 6.5 MPa. Moreover, the prepared scaffolds had appropriate hydrophilicity, weight loss, and blood compatibility. Furthermore, the histopathological findings demonstrated that the defects filled with the PLA/nano-HA scaffolds containing 25 mg nandrolone healed better compared to the other study groups. Conclusion: Therefore, it was concluded that the scaffolds containing nandrolone could be used in bone regeneration. © Mashhad University of Medical Sciences. All Rights Reserved.

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Style	Citing Format
MLA	Salehi M, et al.. "In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects." Nanomedicine Journal, vol. 7, no. 2, 2020, pp. 115-123.
APA	Salehi M, Ai A, Ehterami A, Einabadi M, Taslimi A, Ai A, Akbarzadeh H, Ameli GJ, Farzamfar S, Shirian S, Azimi N, Sadeghi F, Bahrami N, Goodarzi A, Ai J (2020). In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects. Nanomedicine Journal, 7(2), 115-123.
Chicago	Salehi M, Ai A, Ehterami A, Einabadi M, Taslimi A, Ai A, Akbarzadeh H, et al.. "In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects." Nanomedicine Journal 7, no. 2 (2020): 115-123.
Harvard	Salehi M et al. (2020) 'In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects', Nanomedicine Journal, 7(2), pp. 115-123.
Vancouver	Salehi M, Ai A, Ehterami A, Einabadi M, Taslimi A, Ai A, et al.. In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects. Nanomedicine Journal. 2020;7(2):115-123.
BibTex	@article{ author = {Salehi M and Ai A and Ehterami A and Einabadi M and Taslimi A and Ai A and Akbarzadeh H and Ameli GJ and Farzamfar S and Shirian S and Azimi N and Sadeghi F and Bahrami N and Goodarzi A and Ai J}, title = {In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects}, journal = {Nanomedicine Journal}, volume = {7}, number = {2}, pages = {115-123}, year = {2020} }
RIS	TY - JOUR AU - Salehi M AU - Ai A AU - Ehterami A AU - Einabadi M AU - Taslimi A AU - Ai A AU - Akbarzadeh H AU - Ameli GJ AU - Farzamfar S AU - Shirian S AU - Azimi N AU - Sadeghi F AU - Bahrami N AU - Goodarzi A AU - Ai J TI - In Vitro and in Vivo Investigation of Poly(Lactic Acid)/Hydroxyapatite Nanoparticle Scaffold Containing Nandrolone Decanoate for the Regeneration of Critical-Sized Bone Defects JO - Nanomedicine Journal VL - 7 IS - 2 SP - 115 EP - 123 PY - 2020 ER -

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