Selenium Nanoparticle-Enriched Lactobacillus Plantarum Causes More Anti-Carcinogenic Effect on Human Colon Cancer Cells Compared to Non-Enriched Ones Publisher



Zare H¹ ; Sanaei M¹ ; Yazdi MH^{1, 2} ; Shahverdi AR^{1, 2} Show Affiliations
Source: BioNanoScience Published:2023

Abstract

The immunomodulatory effects of Lactobacillus plantarum cell lysate have been demonstrated previously. The purpose of this research is to evaluate the anti-cancer and immunomodulatory effects of non-enriched and selenium nanoparticles-enriched L. plantarum PTCC 1058 cell lysate on the Caco2 cell line. Lactobacillus plantarum cell lysate were added in different concentrations of 100, 50, and 25 µg/mL onto the Caco2 cells and was then followed by a 24-h culture. The MTT assay was used to determine the anti-carcinogenic effect. The results indicated that in both cases, lower concentrations of cell lysate had a greater inhibitory effect on Caco2 cells’ proliferation. However, this effect was significantly stronger in SeNP-enriched cell lysate treated Caco2. We also evaluated the expression of several cytokines in Caco2 cells following 24 h of exposure to 25 µg/mL of non-enriched and SeNP-enriched cell lysate by RT-qPCR. The results demonstrated that both types of cell lysate significantly enhanced IL-12, IFN-γ, and TNF-α expression (P ˂ 0.0001), as well as decreased IL-8, IL-10, and TGF-β expression. It was observed that the magnitudes of these effects were more pronounced for the enriched lysate, suggest that SeNP-enriched L. plantarum cell lysate may be effective in bolstering the immune responses of cancer patients. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.