Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Decreased Fertilization: Human Sperm Dna Fragmentation and in Vitro Maturation of Oocyte in Stimulated Icsi Cycles Publisher



Haghpanah T1 ; Eslamiarshaghi T2 ; Afarinesh MR3 ; Salehi M4, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Kerman University of Medical Sciences, Faculty of Medicine, Dept. of Anatomy, Afzalipour, Kerman, Iran
  2. 2. Shahid Beheshti University of Medical Sciences, Stem Cell Technology Research Center, Dept. of Transgenic Animal Science, Tehran, Iran
  3. 3. Kerman University of Medical Sciences, Institute of Neuropharmacology, Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Kerman, Iran
  4. 4. Cellular and Molecular Biology Research Center, Dept. of Biotechnology, Tehran, Iran
  5. 5. School of Advanced Technologies in Medicine, Dept. of Biotechnology, Tehran, Iran

Source: Acta Endocrinologica Published:2017


Abstract

Objective. The aim of this study was to evaluate the impact of sperm DNA fragmentation (SDF) on fertilization rate, and sperm nuclear decondensation after intracytoplasmic injection of sperm (ICSI) into cumulus-free germinal vesicle (GV) oocytes from stimulated cycles. Methods and study design. After the retrieval of oocytes, the GV oocytes were cultured for 24 h. Oocytes that liberated polar bodies were injected with processed semen, and were used to evaluate SDF level. Based on SDF, the data were categorized into two groups. Group I in which SDF ≤ 30% and group II in which SDF > 30%. Fertilization (presence of two pronuclei) was checked 16-19 h after ICSI. Unfertilized oocytes were stained by Hoechst 33258 and examined to evaluate the undecondensed sperm head in oocyte. The rates of maturation, fertilization in fertilized IVM oocytes and the percentage of undecondensed sperm in the unfertilized oocytes were assessed according to SDF. Results. Out of 146 GV oocytes that were subjected to IVM, 101 (69 %) developed to metaphase II. The fertilization rate of IVM oocytes in group II was significantly lower than that in group I (P < 0.05). Moreover, group I, had 25 % of their unfertilized oocytes containing condensed sperm, while group II had a significantly higher number (53 %) of unfertilized oocytes containing condensed sperm (P < 0.05). Conclusion. SDF had a negative effect on the rate of fertilization in matured in vitro GV oocytes and could lead to an increase in the percentage of undecondensed sperm in IVM oocytes from stimulated cycles. © 2017, Acta Endocrinologica Foundation. All rights reserved.
Related Docs
1. Impact of Sperm Dna Fragmentation on Icsi Outcome and Incidence of Apoptosis of Human Pre-Implantation Embryos Obtained From in Vitro Matured Mii Oocytes, Biochemical and Biophysical Research Communications (2019)
2. Does Sperm Dna Fragmentation Affect the Developmental Potential and the Incidence of Apoptosis Following Blastomere Biopsy?, Systems Biology in Reproductive Medicine (2016)
3. Upregulation of Toll-Like Receptor 4 Through Anti-Mir-Let-7A Enhances Blastocyst Attachment to Endometrial Cells in Mice, Journal of Cellular Physiology (2020)
4. Male Factors: The Role of Sperm in Preimplantation Embryo Quality, Reproductive Sciences (2021)
5. Controversy and Consensus on the Management of Elevated Sperm Dna Fragmentation in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations, World Journal of Men's Health (2023)
Experts (# of related papers)
Hamed Akhavizadegan (1)
Fardin Amidi (1)