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Leukemia Inhibitory Factor’S Effect on the Growth and Survival of Sheep’S Follicles of Ovarian Tissue During Vitrification Publisher Pubmed



Mohammadzadeh F1 ; Ajdary M2 ; Mohammadzadeh A3 ; Safdarian L4 ; Amidi F1 ; Sobhani A1
Authors
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Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Reproductive Endocrinology and Embryology, Reproductive Biotechnology Research Center, Avesina Research Institute, ACECR, Tehran, Iran
  4. 4. Department of Gynecology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Cell and Tissue Banking Published:2023


Abstract

One of the experimental programs for fertility protection in women includes protective cryopreservation. Vitroficasion of ovarian tissue is one of the protective cryopreservation methods that use high concentrations of antifreeze and faster cooling. To reduce its complications, LIF (Leukemia inhibitory factor) was used as a pretreatment in this study. In this study, the ovaries were randomly divided into 8 groups. In NCN (without pretreatment and LIF in culture media), NCP (without pretreatment and with LIF in culture media), PCP (with pretreatment and LIF in culture media), and PCN (with pretreatment and without LIF in culture media) groups, vitrification and reversal were not performed. In the groups NVN (without pretreatment and LIF in culture media), NVP (without pretreatment and with LIF in culture media) PV, PVP (with pretreatment and LIF in culture media), and PVN (with pretreatment and without LIF in culture medium) groups, vitrification and tissue reversal were performed. All groups were cultured and histological, cellular, and molecular evaluations were performed. The results of the present study showed that LIF in the culture medium reduced the number of abnormal, primordial, primary, and secondary follicles, and DNA breakage compared to the group without LIF (P < 0.05) and increases the growth of follicles and expression of GDF9, BMP, AMH, KITLG genes (P < 0.05). The use of LIF pretreatment before vitrification and melting of sheep ovary tissue in its culture medium reduces the damage caused by it and increases the growth and development of ovarian follicles while maintaining their function. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.