Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
A Comparative Study of the Bone Marrow and Umbilical Cord-Derived Mesenchymal Stem Cells (Mscs) Efficiency on Generating Msc-Educated Macrophages (Mems) Publisher Pubmed



Shirin M1, 2 ; Agharezaeei M1, 2 ; Alizadeh S2 ; Bashash D3 ; Sheikhsaran F1 ; Chahardouli B1 ; Mousavi SA1 ; Ahmadvand M4 ; Ghaffari SH1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Science, Tehran, Iran
  2. 2. Department of Hematology, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Cell Therapy and Hematopoietic Stem Cell Transplantation Research Center, Research Institute for Oncology, Hematology and Cell Therapy, Tehran University of Medical Sciences, Tehran, Iran

Source: Asian Pacific Journal of Cancer Prevention Published:2022


Abstract

Background: Mesenchymal stem cells (MSCs) have gained much more attention in cell therapy and regenerative medicine due to their immunosuppressive effects. MSCs have interaction with other immune cells, such as macrophages (MQs). Bone marrow (BM)-derived MSCs can educate MQs toward MSC-educated MQs (MEMs) which possess an anti-inflammatory immunophenotype. Given this and based on the important limitations of BM collection, we hypothesized whether co-culture of MQs with umbilical cord (UC)-derived MSCs can result in the MEM phenotype. Methods: First, isolated monocytes cultured for five days to obtain M0 MQs. Then, they were co-cultured with either BM or UC-MSCs under direct and indirect conditions. After three days of co-culture, MEM-specific surface markers, as well as the gene expression of inflammatory and anti-inflammatory cytokines, were evaluated. Results: Surface expression of CD163/CD206, as specific markers for M2 MQs, increased in MEMs after co-culture with BM and UC-derived MSCs, while CD80/CD86 expression (specific markers for M1 MQs) didn’t change significantly. The mRNA expressions of PDL-1 as well as anti-inflammatory cytokines, including IL-6, IL-10, and TGFβ also increased in MEMs after co-culture of UC-MSCs compared to control MQs (p <.05), while the expression of IL-12 was significantly decreased (p<.001). Conclusions: To the best of our knowledge, this study shows for the first time that the co-culture of MQs with UC-derived MSCs efficiently contributes to the generation of MEMs even greater than BM-MSCs; shedding light on the promising potential of UC as an alternative source to educate MQs in vitro. © This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License.
Related Docs
View other Related Docs
1. Modulation of Macrophage Polarization for Bone Tissue Engineering Applications, Iranian Journal of Allergy# Asthma and Immunology (2018)
2. Liothyronine Could Block the Programmed Death-Ligand 1 (Pdl1) Activity: An E-Pharmacophore Modeling and Virtual Screening Study, Journal of Receptors and Signal Transduction (2022)
3. Genetically Engineered Mesenchymal Stromal Cells As a New Trend for Treatment of Severe Acute Graft-Versus-Host Disease, Clinical and Experimental Immunology (2022)
4. Effect of Efferocytosis of Apoptotic Mesenchymal Stem Cells (Mscs) on C57bl/6 Peritoneal Macrophages Function, Life Sciences (2018)
5. Therapeutic and Immunomodulatory Potentials of Mesenchymal Stromal/Stem Cells and Immune Checkpoints Related Molecules, Biomarker Research (2024)
6. Pd-1/Pd-L1 Pathway: Basic Biology and Role in Cancer Immunotherapy, Journal of Cellular Physiology (2019)
7. Pd-1/Pd-L and Autoimmunity: A Growing Relationship, Cellular Immunology (2016)
8. Mesenchymal Stem Cells: Rising Concerns Over Their Application in Treatment of Type One Diabetes Mellitus, Journal of Diabetes Research (2015)
Experts (# of related papers)
View all Related Experts
Nima Rezaei (2)
Sara Assadiasl (2)
Other Related Docs
9. The Emerging Crosstalk Between Atherosclerosis-Related Micrornas and Bermuda Triangle of Foam Cells: Cholesterol Influx, Trafficking, and Efflux, Cellular Signalling (2023)
10. Monocyte-Derived and M1 Macrophages From Ankylosing Spondylitis Patients Released Higher Tnf-Α and Expressed More Il1b in Response to Bzatp Than Macrophages From Healthy Subjects, Scientific Reports (2021)
11. Immune Checkpoint Inhibitor (Ici) Therapy in Central Nervous System Cancers: State-Of-The-Art and Future Outlook, International Immunopharmacology (2025)
12. A Review of Preclinical Experiments Toward Targeting M2 Macrophages in Prostate Cancer, Current Drug Targets (2019)
13. The Sdldl Reduces Mrc1 Expression Level and Secretion of Histamin E in Differentiated M2-Macrophages From Patients With Coronary Artery Stenosis, Cardiovascular and Hematological Disorders - Drug Targets (2017)
14. Immunotherapy a New Hope for Cancer Treatment: A Review, Pakistan Journal of Biological Sciences (2018)
15. Ankylosing Spondylitis and Mesenchymal Stromal/Stem Cell Therapy: A New Therapeutic Approach, Biomedicine and Pharmacotherapy (2019)
16. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application, Frontiers in Cell and Developmental Biology (2020)
17. Challenges in Mesenchymal Stromal Cell-Based Therapies, Current Stem Cell Research and Therapy (2023)
18. Preclinical and Clinical Studies on the Use of Extracellular Vesicles Derived From Mesenchymal Stem Cells in the Treatment of Chronic Wounds, Molecular Pharmaceutics (2024)
19. Covid-19 Immunotherapy: Treatment Based on the Immune Cell-Mediated Approaches, International Immunopharmacology (2022)
20. Pd-1 and Cancer: Molecular Mechanisms and Polymorphisms, Immunogenetics (2018)
21. Mesenchymal Stem Cell As a Novel Approach to Systemic Sclerosis; Current Status and Future Perspectives, Cell Regeneration (2020)