Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes

Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes Publisher Pubmed

Pourdashti S¹ ; Faridi N¹ ; Yaghooti H² ; Jalali MT³ ; Soroush A⁴ ; Bathaie SZ¹

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1. Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University (TMU), Tehran, Iran
2. Cellular and Molecular Research Center, Hyperlipidemia Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
3. Hyperlipidemia Research Center, Diabetes Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
4. Obesity and Eating Habits Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnic and Histochemistry Published:2022

Abstract

Regulation of angiogenesis plays an important role in adipose tissue expansion and function. The Wnt pathway and WNT10B, the main member of Wnt family, participate in angiogenesis in cancer tumors, but there is limited evidence to support the regulatory role of WNT10B in human adipose tissue angiogenesis. Subcutaneous white adipose tissue (scWAT) of 80 participants including obese and non-obese subjects was obtained and the expression of WNT10B and VEGFA genes were evaluated using qPCR. Human adipose-derived stem cells (hADSC) were differentiated to adipocytes and incubated under either hypoxic or normoxic conditions. The conditioned media of these adipocytes were collected and used as growth media for human umbilical vein endothelial cells (HUVEC) in Matrigel. We evaluated the proliferation, cell cycle phases, tubule formation and β-catenin activation of these treated cells. We found a significant correlation between WNT10B and VEGFA expression in the scWAT of both obese and non-obese subjects. Proliferation and tubule formation of HUVEC treated with conditioned media of hypoxic adipocytes (hCM) in the S-phase were increased significantly compared to the HUVEC treated with the conditioned media of normoxic adipocytes (nCM). The expression of WNT10B and VEGFA was enhanced in hypoxic adipocytes compared to normoxic adipocytes; also, activation and nuclear translocation of β-catenin was enhanced in the HUVEC treated with hCM compared to nCM. WNT10B acts as an angiogenic protein in scWAT under hypoxic conditions. Hypoxia induced WNT10B increases VEGFA expression and causes tube formation by HUVECs and angiogenesis in adipose tissue via the canonical Wnt/β-catenin pathway. © 2021 The Biological Stain Commission.

2. Inflammatory Cytokines and Type 2 Diabetes, Koomesh (2020)

3. Molecular and Cellular Mechanisms Linking Inflammation to Insulin Resistance and Β-Cell Dysfunction, Translational Research (2016)

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Ahmadreza Soroush (1)

Ghodratollah Panahi (1)

Style	Citing Format
MLA	Pourdashti S, et al.. "Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes." Biotechnic and Histochemistry, vol. 97, no. 3, 2022, pp. 168-179.
APA	Pourdashti S, Faridi N, Yaghooti H, Jalali MT, Soroush A, Bathaie SZ (2022). Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes. Biotechnic and Histochemistry, 97(3), 168-179.
Chicago	Pourdashti S, Faridi N, Yaghooti H, Jalali MT, Soroush A, Bathaie SZ. "Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes." Biotechnic and Histochemistry 97, no. 3 (2022): 168-179.
Harvard	Pourdashti S et al. (2022) 'Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes', Biotechnic and Histochemistry, 97(3), pp. 168-179.
Vancouver	Pourdashti S, Faridi N, Yaghooti H, Jalali MT, Soroush A, Bathaie SZ. Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes. Biotechnic and Histochemistry. 2022;97(3):168-179.
BibTex	@article{ author = {Pourdashti S and Faridi N and Yaghooti H and Jalali MT and Soroush A and Bathaie SZ}, title = {Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes}, journal = {Biotechnic and Histochemistry}, volume = {97}, number = {3}, pages = {168-179}, year = {2022} }
RIS	TY - JOUR AU - Pourdashti S AU - Faridi N AU - Yaghooti H AU - Jalali MT AU - Soroush A AU - Bathaie SZ TI - Possible Role of Wnt10b in Increased Proliferation and Tubule Formation of Human Umbilical Vein Endothelial Cell Cultures Treated With Hypoxic Conditioned Medium From Human Adipocytes JO - Biotechnic and Histochemistry VL - 97 IS - 3 SP - 168 EP - 179 PY - 2022 ER -

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