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Evaluation of Re and B1 Genes As Targets for Detection of Toxoplasma Gondii by Nested Pcr in Blood Samples of Patients With Ocular Toxoplasmosis Publisher Pubmed



Rahimi Esboei B1 ; Kazemi B2 ; Zarei M3 ; Mohebali M1 ; Keshavarz Valian H1 ; Shojaee S1 ; Zahedipour F2 ; Fallahi S4 ; Mousavi P1 ; Mahmoudzadeh R3 ; Salabati M3
Authors
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Authors Affiliations
  1. 1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Eye Research Center, Farabi Eye Hospital, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran

Source: Acta Parasitologica Published:2019


Abstract

Introduction: To evaluate B1 and RE genes as targets to detect Toxoplasma gondii, nested PCR is used in blood samples of patients with ocular toxoplasmosis. Materials and Methods: Following the measurement of IgG and IgM antibodies using indirect ELISA, IgG avidity and assessment of blood samples by nested PCR, the agreement between various test results was studied. Results: From 117 patients, 77 (65.81%) were found to be positive for IgG anti-Toxoplasma antibody, 12 cases were positive for both IgG and IgM, and 1 patient was positive for IgM only. The detection limit for the RE-nested PCR assay was one T. gondii tachyzoite, whereas the limit for B1-nested PCR was five tachyzoites. Nested PCR results showed higher agreement with IgM test results than IgG test results. Conclusion: The results of this study showed that nested PCR of peripheral blood is a useful and non-invasive method for detection of T. gondii in patients with OT, especially in case of recently acquired infections, and RE targeted assay is more sensitive than B1 targeted assay for this purpose. © 2019, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences.
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