Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Genotoxic and Cytotoxic Effects of Some Insecticides Used in Iran on Murine Fibroblast Cells (L-929) Publisher



Mohseni H1 ; Sahebnasagh R2 ; Tavajohi S1 ; Ghahremani MH1 ; Kebriaeezadeh A1 ; Aliebrahimi S3 ; Ostad SN4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Education, Virtual University of Medical Sciences, Tehran, Iran
  4. 4. Toxicology and Poisoning Research Centre, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Toxicology and Environmental Health Sciences Published:2022


Abstract

Objective: Insecticides play an essential role in preventing crop losses. However, there is growing concern over adverse effects associated with their widespread and long-term use. Cypermethrin (CYP) and lambda-cyhalothrin (LCT) are type II pyrethroid insecticides that inhibit voltage-sensitive sodium channel function. Emamectin benzoate (EMB) is an avermectin class insecticide that stimulates γ-aminobutyric acid (GABA) receptor and glutamate-gated chloride channel. Methods: The L-929 cells were exposed to CYP, LCT, and EMB for 48 h to measure the cytotoxic effect by MTT assay. Then, Annexin V/PI was used to evaluate apoptosis. Finally, Comet assay was used to determine the genotoxicity and DNA damage. Results: We present the investigation results of potential cytotoxic and genotoxic effects of LCT, CYP, and EMB insecticides on normal murine fibroblast cells (L-929). Our results indicated that cell viability decreased with increasing concentrations of insecticides during 48 h. Moreover, the apoptosis induction by EMB was more potent than LCT and CYP. The comet assay findings indicated that CYP causes more DNA damage than the other two insecticides. As a result, CYP may be considered a genotoxic insecticide. Conclusion: Our results may be included in future research to provide a more thorough assessment of the toxicity of these insecticides. © 2022, The Author(s), under exclusive licence to Korean Society of Environmental Risk Assessment and Health Science.