Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine

Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine Publisher Pubmed

Borghei YS¹ ; Hosseini M^{1, 2} ; Khoobi M^{3, 4} ; Ganjali MR^{5, 6}

Show Affiliations

1. Department of Life Science Engineering, University of Tehran, Tehran, Iran
2. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Pharmaceutical Biomaterials and Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran
4. Nanobiomaterials Group, Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, Iran
5. Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran
6. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Luminescence Published:2017

Abstract

A remarkable method for the highly sensitive detection of phenylalanine and tryptophan based on a chemiluminescence (CL) assay was reported. It was found that fluorescent copper nanoclusters capped with cysteine (Cys-CuNCs) strongly enhance the weak CL signal resulting from the reaction between luminol and H2O2. Of the amino acids tested, phenylalanine and tryptophan could enhance the above CL system sensitively. Under optimum conditions, this method was satisfactorily described by a linear calibration curve over a range of 1.0 × 10−6 to 2.7 × 10−5 M for phenylalanine and 1.0 × 10−7 to 3.0 × 10−5 M for tryptophan, respectively. The effect of various parameters such as Cys-CuNC concentration, H2O2 concentration and pH on the intensity of the CL system were also studied. The main experimental advantage of the proposed method was its selectivity for two amino acids compared with others. To evaluate the applicability of the method to the analysis of a real biological sample it was used to determine tryptophan and phenylalanine in human serum and remarkable results were obtained. Copyright © 2017 John Wiley & Sons, Ltd.

Style	Citing Format
MLA	Borghei YS, et al.. "Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine." Luminescence, vol. 32, no. 6, 2017, pp. 1045-1050.
APA	Borghei YS, Hosseini M, Khoobi M, Ganjali MR (2017). Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine. Luminescence, 32(6), 1045-1050.
Chicago	Borghei YS, Hosseini M, Khoobi M, Ganjali MR. "Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine." Luminescence 32, no. 6 (2017): 1045-1050.
Harvard	Borghei YS et al. (2017) 'Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine', Luminescence, 32(6), pp. 1045-1050.
Vancouver	Borghei YS, Hosseini M, Khoobi M, Ganjali MR. Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine. Luminescence. 2017;32(6):1045-1050.
BibTex	@article{ author = {Borghei YS and Hosseini M and Khoobi M and Ganjali MR}, title = {Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine}, journal = {Luminescence}, volume = {32}, number = {6}, pages = {1045-1050}, year = {2017} }
RIS	TY - JOUR AU - Borghei YS AU - Hosseini M AU - Khoobi M AU - Ganjali MR TI - Copper Nanocluster-Enhanced Luminol Chemiluminescence for High-Selectivity Sensing of Tryptophan and Phenylalanine JO - Luminescence VL - 32 IS - 6 SP - 1045 EP - 1050 PY - 2017 ER -

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