A Sensitive Signal-On Electrochemiluminescence Sensor Based on a Nanocomposite of Polypyrrole-Gd2o3 for the Determination of L-Cysteine in Biological Fluids Publisher Pubmed



Karimi A¹ ; Husain SW¹ ; Hosseini M^{2, 3} ; Azar PA¹ ; Ganjali MR^{4, 5} Show Affiliations
Source: Microchimica Acta Published:2020

Abstract

A sensitive solid-state electrochemiluminescence (ECL) electrode for L-cysteine was developed based on depositing layers of a hybrid nanocomposite of polypyrrole-dodecyl benzene sulfate-sodium perchlorate-sodium carbonate-gadolinium (PPy-Gd2O3) on a platinum substrate. The presence of the Gd2O3 nanoparticle layer improved the ECL signal, and under optimum conditions, a linear relationship was observed between the signal and the logarithm of L-cysteine concentration from 1.0 × 10−13 to 1.0 × 10−6 M (R2 = 0.9937). At the emission wavelength around 425 nm , at which the analytical signal was measured, the electrode showed an RSD of less than 4% and a low detection limit of 4.2 × 10−14 M. The results proved to be reproducible and stable, and the electrode was applicable in the determination of L-cysteine in biological fluids with recoveries from 94.0–107%. [Figure not available: see fulltext.] © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.