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Pentoxifylline Promotes Spermatogenesis Via Upregulation of the Nrf2-Are Signalling Pathway in a Mouse Model of Germ-Cell Apoptosis Induced by Testicular Torsion-Detorsion Publisher Pubmed



Akanji OD1 ; Hassanzadeh G2 ; Malekzadeh M3 ; Khanmohammadi N2 ; Khanezad M3 ; Sadeghiani G2 ; Rastegar T2, 4
Authors
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Authors Affiliations
  1. 1. Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria
  2. 2. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Anatomy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Burns and Regenerative Medicine Research Center, Guilan University of Medical Sciences, Rasht, Iran

Source: Reproduction# Fertility and Development Published:2023


Abstract

Context: Testicular torsion-detorsion results in loss of germ cells and infertility. Pentoxifylline has been shown to prevent tissue damage. Aims: To determine the effect of pentoxifylline on germ cell survival in torsion-detorsion induced apoptosis Methods: Twenty male mice were divided into four groups of five animals each: Control, T1 (Torsion-detorsion + single dose 100 mg/kg Pentoxifylline/IP), T2 (Torsion-detorsion + daily 20 mg/kg pentoxifylline/IP for 2 weeks, and T/D (Torsion-detorsion only). 35th day after torsion-detorsion, the left testes of all the animals were harvested for histological and biochemical analysis. Key results: Histomorpholoical analysis showed significant increase (P < 0.05) in seminiferous tubule diameter, Johnsen's score and germ cells of Control and T1 compared to T2 and T/D, with no significant difference (P > 0.05) in testis weight, sertoli, leydig and myoid cells. Tunnel assay showed significant increase (P < 0.05) in apoptotic cells of T/D and T2 animals compared to Control and T1. RT-PCR analysis showed significant high (P < 0.01) mRNA expression of Bax gene in T/D compared to T1 and T2 and significant increase (P < 0.05) of Bcl2 in Control, T1, T2 compared to T/D. Nrf2-ARE transcripts revealed significant increase (P < 0.05) in Control and T1 compared to T2 and T/D. Western blot showed significantly increased (P < 0.05) caspase-3 in T/D compared to Control, T1 and T2. Conclusion: Pentoxifylline promotes spermatogenesis and suppressed apoptosis induced by testicular torsion-detorsion. Implication: Pentoxifylline could serve as adjunct therapy to surgery in the treatment of torsion-detorsion induced germ cell apoptosis. © 2023 CSIRO. All rights reserved.
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