Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion

Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion Publisher

Dhulqarnain AO¹ ; Takzaree N^{1, 2} ; Hassanzadeh G^{1, 2} ; Tooli H^{3, 5} ; Malekzadeh M² ; Khanmohammadi N² ; Yaghobinejad M² ; Solhjoo S⁴ ; Rastegar T^{1, 2}

Show Affiliations

1. Department of Anatomy, School of Medicine, International Campus, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Anatomy, School of Medicine, Shahroud University of Medical Sciences, Sharoud, Iran
4. Department of Anatomy, Kerman University of Medical Sciences, Kerman, Iran
5. Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran

Source: Heliyon Published:2021

Abstract

Testicular torsion-detorsion results in enhanced formation of free radicals which contribute to the pathophysiology of testicular tissue damage. Recent reports have identified protective role of pentoxifylline (PTX) against free radicals. Thus, we determined the protective effect of pentoxifylline against testicular damage in mouse model of testicular torsion-detorsion. Twenty (6 weeks old) male mice were divided into 4 groups of 5 animals each namely: Control (sham operated group), T1 (Torsion-detosion + single dose 100 mg/kg PTX, T2 (torsion-detorsion + 20 mg/kg PTX for 2 weeks and T/D (torsion-detorsion only). Animals in T1, T2 and T/D groups underwent 2 h of testicular torsion with the left testes rotated 720° (clockwisely) followed by 30 min of detorsion. After detorsion, drug administration was done intraperitoneally. The left testes of all the animals were excised on the 35th day after torsion-detortion for histopathological and biochemical assay. Histomorphological analysis of the seminiferous tubules showed that there were significant increase (P < 0.01 or 0.05) in the mean seminiferous tubule diameter, Johnson score and germ cells of animals in Control and T1 compared to T2 and T/D with no significant difference (P > 0.05) in testes weight, sertoli, leydig and myoid cells in all groups. IHC results showed significant increase (P < 0.01 or 0.05) in id4 and scp3 protein markers in Control, T1 and T2 compared to T/D. Oxidative stress analysis revealed that Pentoxifylline significantly increased (P < 0.01 or 0.05) the level of SOD, catalase, mRNA expression of akt and pi3k genes but significantly suppress (P < 0.01 or 0.05) MDA and Caspase-3 level in Control, T1 and T2 compared to T/D. Pentoxifylline could be used as an adjunct therapy to surgery in the treatment of torsion-detorsion related testicular injury, However, Further studies are needed to evaluate the effects of pentoxifylline on testicular torsion. © 2021

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Style	Citing Format
MLA	Dhulqarnain AO, et al.. "Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion." Heliyon, vol. 7, no. 4, 2021, pp. -.
APA	Dhulqarnain AO, Takzaree N, Hassanzadeh G, Tooli H, Malekzadeh M, Khanmohammadi N, Yaghobinejad M, Solhjoo S, Rastegar T (2021). Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion. Heliyon, 7(4), -.
Chicago	Dhulqarnain AO, Takzaree N, Hassanzadeh G, Tooli H, Malekzadeh M, Khanmohammadi N, Yaghobinejad M, Solhjoo S, Rastegar T. "Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion." Heliyon 7, no. 4 (2021): -.
Harvard	Dhulqarnain AO et al. (2021) 'Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion', Heliyon, 7(4), pp. -.
Vancouver	Dhulqarnain AO, Takzaree N, Hassanzadeh G, Tooli H, Malekzadeh M, Khanmohammadi N, et al.. Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion. Heliyon. 2021;7(4):-.
BibTex	@article{ author = {Dhulqarnain AO and Takzaree N and Hassanzadeh G and Tooli H and Malekzadeh M and Khanmohammadi N and Yaghobinejad M and Solhjoo S and Rastegar T}, title = {Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion}, journal = {Heliyon}, volume = {7}, number = {4}, pages = {-}, year = {2021} }
RIS	TY - JOUR AU - Dhulqarnain AO AU - Takzaree N AU - Hassanzadeh G AU - Tooli H AU - Malekzadeh M AU - Khanmohammadi N AU - Yaghobinejad M AU - Solhjoo S AU - Rastegar T TI - Pentoxifylline Improves the Survival of Spermatogenic Cells Via Oxidative Stress Suppression and Upregulation of Pi3k/Akt Pathway in Mouse Model of Testicular Torsion-Detorsion JO - Heliyon VL - 7 IS - 4 SP - EP - PY - 2021 ER -

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