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Investigation of the Role of Il18, Il-1Β and Nlrp3 Inflammasome in Reducing Expression of Flg-2 Protein in Psoriasis Vulgaris Skin Lesions Publisher Pubmed



Akhlaghi M1 ; Karrabi M2 ; Atabti H3 ; Raoofi A4, 5 ; Mousavi Khaneghah A6
Authors
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Authors Affiliations
  1. 1. Student Research Committee, Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
  2. 2. Cellular and Molecular Research Center, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
  3. 3. Department of Immunology and Biology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
  5. 5. Department of Anatomy, Sabzevar University of Medical Sciences, Sabzevar, Iran
  6. 6. Department of Food Science and Nutrition, Faculty of Food Engineering, University of Campinas (UNICAMP), Campinas, Brazil

Source: Biotechnic and Histochemistry Published:2022


Abstract

We investigated the effects of the NACHT leucine-rich repeat- and PYD-containing proteins (NLRP3) inflammasome, interleukin −18 (IL-18) and interleukin-1 beta (IL-1β) cytokines on the expression of filaggrin-2 (FLG-2) protein in psoriasis patients. Peripheral blood mononuclear cells (PBMC), including T cells, were isolated from psoriasis patients and healthy donors. Ribonucleic acid (RNA) extraction and reverse transcription-polymerase chain reaction (RT-PCR) were performed for all specimens. Immunohistochemical analysis for FLG-2 in normal and psoriatic epidermal tissue also was performed. Western blot was used to separate and identify FLG-2 protein, and immunohistochemical staining was performed to assess FLG-2 expression for psoriasis skin lesions and normal skin. RT-PCR analysis indicated that NLRP3 inflammasome, IL-18 cytokine and IL‐1β cytokine expression were increased in psoriatic epidermis compared to normal skin. We found that the expression of FLG-2 was decreased in psoriatic epidermis compared to normal skin. Higher levels of NLRP3 help decrease the FLG-2 level. © 2021 The Biological Stain Commission.
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