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Molecular Detection of Leishmania Dna in Wild-Caught Sand Flies, Phlebotomus and Sergentomyia Spp. in Northern Iran Publisher



Nikookar SH1 ; Akbari MR2 ; Oshaghi MA3 ; Hosseinivasoukolaei N4 ; Enayati A4 ; Motevallihaghi F4 ; Fakhar M5, 6
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Authors Affiliations
  1. 1. Health Sciences Research Center, Department of Medical Entomology and Vector Control, School of Public Health, Mazandaran University of Medical Sciences, Sari, Iran
  2. 2. MSc Student Research Committee, School of Public Health, Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Medical Entomology and Vector Control, School of Public Health, Health Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  5. 5. Iranian National Registry Center for Lophomoniasis and Toxoplasmosis, Toxoplasmosis Research Center, Imam Khomeini Hospital, Mazandaran University of Medical Sciences, Sari, Iran
  6. 6. Department of Medical Microbiology and Immunology, School of Medicine, Qom University of Medical Sciences, Qom, Iran

Source: Parasite Epidemiology and Control Published:2024


Abstract

Leishmaniasis is currently considered a major health problem in Iran, posing an increasing threat to society's development in various dimensions. This study aimed to detect Leishmania infection in wild-caught sand flies in Sari City, northern Iran. Sand flies were collected using sticky traps, and Leishmania DNA was identified using polymerase chain reaction (PCR) targeting the ITS2-rDNA region, followed by restriction fragment length polymorphism (RFLP) analysis. A total of 138 female sand flies were tested, among which, only 1 specimen of Ph. papatasi (11.11 %) and Ph. major (14.28 %), 4 specimens of Ph. kandelakii (7.27 %) and Se. dentata (8.33 %), and 2 specimens of Se. sintoni (50 %) were naturally infected with L. (L.) major. This was observed in the ITS2 nested-PCR amplification assays where a ∼ 245 bp PCR band was produced. Also, RFLP analysis by Mnl1 revealed the fragments of 55 and 70 and 120 bp for infected sand flies which are characteristic of L. (L.) major. Most of the sand flies were unfed, collected during warm season, found indoor. This study reperesents the first molecular detection of L. (L.) major in wild-caught sand flies, specifically in Ph. papatasi in this region, as well as Ph. kandelakii and Ph. major in Iran and even the world. © 2024
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