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The Effect of Antimicrobial Photodynamic Therapy on the Expression of Novel Methicillin Resistance Markers Determined Using Cdna-Aflp Approach in Staphylococcus Aureus Publisher Pubmed



Hoorijani MN1, 2 ; Rostami H1 ; Pourhajibagher M1, 3, 4 ; Chiniforush N4 ; Heidari M5 ; Pourakbari B6 ; Kazemian H1 ; Davari K7 ; Amini V8 ; Raoofian R9 ; Bahador A1
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, Kurdestan Science and Research Branch, Islamic Azad University, Sanandaj, Iran
  3. 3. Dental Implant Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Laser Research Center of Dentistry (LRCD), Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Pediatrics Infectious Disease Research Center, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Microbiology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
  8. 8. Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran
  9. 9. Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2017


Abstract

Background Widespread methicillin resistant Staphylococcus aureus (MRSA) and absence of effective antimicrobial agents has led to limited therapeutic options for treating MRSA infection. We aimed to evaluate the effect of antimicrobial photodynamic therapy (aPDT) on the expression of novel identified methicillin resistance markers (NIMRMs) in S. aureus using complementary DNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) approaches to address the therapeutic alternatives for MRSA infections. Materials and methods We used cDNA-AFLP to compare MRSA and methicillin susceptible S. aureus (MSSA) for identification of target genes implicated in methicillin resistance. To determine the sub-lethal aPDT (sPDT), MRSA and MSSA clinical isolates photosensitized with toluidine blue O (TBO), and then were irradiated with diode laser. After sPDT, the colony forming units/mL was quantified. Antimicrobial susceptibility against methicillin was assessed for cell-surviving aPDT. Effects of sPDT on the expression of NIMRMs were evaluated by real-time quantitative reverse transcription PCR. Results According to our results, serine hydrolase family protein (Shfp) encoding gene and a gene encoding a conserved hypothetical protein (Chp) were implicated in methicillin resistance in MRSA. sPDT reduced the minimum inhibitory concentrations of methicillin by 3-fold in MRSA. sPDT could lead to about 10- and 6.2- fold suppression of expression of the Chp and Shfp encoding genes, respectively. Conclusion sPDT would lead to reduction in resistance to methicillin of MRSA in surviving cells by suppressing the expression of the Shfp and Chp encoding genes associated with methicillin resistance. This may have potential implications of aPDT for the treatment of MRSA infections. © 2017 Elsevier B.V.
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