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Immunotherapeutic Efficacy of a Lactobacillus Caseilysate As an Adjuvant Combined With a Heated-4T1mammary Carcinoma Cell Lysate in a Murine Model Ofbreast Cancer Publisher



Dorostkar R1 ; Hashemzadeh MS1 ; Jafari S2 ; Tat M1 ; Ghalavand M1 ; Asghari MH3, 4 ; Moloudizargari M5
Authors
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Authors Affiliations
  1. 1. Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, 14351, Iran
  2. 2. Faculty of Veterinary Medicine, Urmia University, Urmia, 57153, Iran
  3. 3. Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, 47176, Iran
  4. 4. Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Tehran University of Medical Sciences, Tehran, 14155, Iran
  5. 5. Department of Immunology, School of Medicine, Shahid BeheshtiUniversity of Medical Sciences, Tehran, 19839, Iran

Source: Asian Biomedicine Published:2016


Abstract

Background: Immunotherapy, during which the immune system of the patient is manipulated to act againsttumors has been among the most successful methods in the treatment of breast cancer, a leading cause ofmortality among women worldwide.Objectives: To investigate the immunotherapeutic efficacy of Lactobacillus casei lysate as an adjuvant incombination with a heated-4T1 mammary carcinoma cell lysate in a model of breast cancer.Methods: After ethics committee approval of all animal procedures, a murine model of breast cancer was inducedin BALB/c mice using 4T1 cells. These mice were immunized with a combination of lysates of heated 4T1 cellsand L. casei. Subsequent changes in tumor size and weight, and the production of TNF-α, IL-2, IL-12, IL-17, and IL13 were measured. Lung weights were measured as an indicator of metastasis to other organs.Results: The tumor size and weight in mice immunized with the combined vaccine were significantly reducedcompared with controls. The combined immunotherapy altered the pattern of cytokine production to theadvantage of antitumor immunity, and was significantly more potent than immunization with heated-4T1-celllysate or L. casei lysate alone.Conclusions: Coadministration of L. casei lysate enhanced the immunotherapeutic efficacy of the heated-4T1-cell lysate as a source of tumor-associated antigens. L. casei can potentially be used as an adjuvant combinedwith sources of tumor antigens in the treatment of cancers, and as a safe alternative to the current adjuvantsthat cause greater irritation to hosts. Further studies are required to clarify the mechanisms underlying theseeffects.