Radio Frequency Hyperthermia of Cancerous Cells With Gold Nanoclusters: An in Vitro Investigation Publisher



Amini SM¹ ; Kharrazi S¹ ; Jaafari MR² Show Affiliations
Source: Gold Bulletin Published:2017

Abstract

Heating response of gold nanoclusters of two different sizes (2 and 3 nm) under radio frequency electric field has been reported here for the first time. Experiments were performed on colloidal solution of nanoparticles and their in vitro hyperthermia effect was investigated on pancreatic cancer cells (MIA Paca-2). Gold nanoclusters at concentrations up to 200 ppm were found non-toxic, based on the results of cytotoxicity assay using tetrazolium dye MTT and at concentrations up to 50 ppm exhibited very low hemolytic activity. Gold nanoclusters of two sizes resulted in up to 50 °C temperature rise when in radio frequency electric field of 100 W power. Smaller clusters were found more efficient for heat generation and thus were applied in cell studies. Gold nanoclusters (at 5–200 ppm concentrations) under radio frequency electric field (100 W, 2 and 4 min of exposure duration) exhibited successful eradication of pancreatic cancerous cells. © 2017, Springer International Publishing Switzerland.