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Preparation and Characterization of Human Size Whole Heart for Organ Engineering: Scaffold Microangiographic Imaging Publisher Pubmed



Akbarzadeh A1 ; Khorramirouz R1 ; Ghorbani F2 ; Beigi RSH1 ; Hashemi J3 ; Kajbafzadeh AM1
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Authors Affiliations
  1. 1. Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Tracheal Diseases Research Center (TDRC), National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of Medicine, North Khorasan, University of Medical Sciences, Bojnurd, Iran

Source: Regenerative Medicine Published:2019


Abstract

Definitive treatment for end-stage heart failure is heart transplantation, however, this is associated with several limitations. Aim: We decellularized and assessed ovine hearts through coronary perfusion. To evaluate in situ recellularization, a decellular graft was transplanted hetrotopically into the omental wrap. Results: Cell removal was confirmed by DNA count (11.68 ± 3.42 ng/mg dry weight). Elastic, reticular and collagen fiber were well preserved. There was a slight change in both glycosaminoglycan (7.01 ± 1.36 to 8.37 ± 0.32 μg/mg) and collagen (32.37 ± 2.3 to 36.31 ± 2.1) μg/mg (p > 0.05). Angiography and blood circulation revealed an intact vascular network. Implantation led to proper vascularization. Image J indicated CD31: 23.98 ± 12.3; CD34: 48.67 ± 19.5 and αSMA: 78.33 ± 27.8 inch/cm. Conclusion: Bio-scaffold of human size heart is achievable for future steps employing this technique. © 2019 Future Medicine Ltd.
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