Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine

Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine Publisher Pubmed

Pourfarzib M^{1, 2} ; Shekarchi M² ; Rastegar H² ; Akbariadergani B¹ ; Mehramizi A³ ; Dinarvand R^{1, 4}

Show Affiliations

1. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
2. Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran, Iran
3. Tehran Chemie Pharmaceutical Co., Tehran, Iran
4. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences Published:2015

Abstract

A molecularly imprinted polymer (MIP) has been synthesized in order to specifically extract efavirenz from serum and urine by dispersive solid-phase extraction following by HPLC-UV analysis. The imprinted nanoparticles were prepared by miniemulsion polymerization method using efavirenz as template molecule and methacrylic acid as functional monomer. Molecular recognition properties, binding capacity and selectivity of the MIPs were evaluated and the results revealed that the obtained MIPs had high specific retention for efavirenz in aqueous medium. The MIP was used as a molecular sorbent for the separation of efavirenz from human serum and urine. The extraction of efavirenz by MIP coupled with HPLC analysis showed a linear calibration curve in the range of 50-300. μg/L with exellent precisions (3.66% and 4.6% for 100 and 300. μg/L respectively). The limit of detection (LOD) and limit of quantification (LOQ) were determind in serum (17.3 and 57.5. μg/L) and urine (10.6 and 36.2. μg/L). The maximum recoveries for serum and urine samples were found to be 95.2% and 92.7% respectively. Due to the high precision and accuracy, this method may be the UV-HPLC choice with MIP extraction for bioequivalence analysis of efavirenz in serum and urine. © 2014 Elsevier B.V.

Experts (# of related papers)

Ali Mohammadi (1)

Style	Citing Format
MLA	Pourfarzib M, et al.. "Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine." Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 974, no. , 2015, pp. 1-8.
APA	Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R (2015). Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 974(), 1-8.
Chicago	Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R. "Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine." Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 974, no. (2015): 1-8.
Harvard	Pourfarzib M et al. (2015) 'Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 974(), pp. 1-8.
Vancouver	Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R. Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2015;974():1-8.
BibTex	@article{ author = {Pourfarzib M and Shekarchi M and Rastegar H and Akbariadergani B and Mehramizi A and Dinarvand R}, title = {Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine}, journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}, volume = {974}, number = {}, pages = {1-8}, year = {2015} }
RIS	TY - JOUR AU - Pourfarzib M AU - Shekarchi M AU - Rastegar H AU - Akbariadergani B AU - Mehramizi A AU - Dinarvand R TI - Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences VL - 974 IS - SP - 1 EP - 8 PY - 2015 ER -

Style

Citing Format

MLA

Pourfarzib M, et al.. "Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine." Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 974, no. , 2015, pp. 1-8.

APA

Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R (2015). Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 974(), 1-8.

Chicago

Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R. "Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine." Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 974, no. (2015): 1-8.

Harvard

Pourfarzib M et al. (2015) 'Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 974(), pp. 1-8.

Vancouver

Pourfarzib M, Shekarchi M, Rastegar H, Akbariadergani B, Mehramizi A, Dinarvand R. Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2015;974():1-8.

BibTex

@article{ author = {Pourfarzib M and Shekarchi M and Rastegar H and Akbariadergani B and Mehramizi A and Dinarvand R}, title = {Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine}, journal = {Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences}, volume = {974}, number = {}, pages = {1-8}, year = {2015} }

RIS

TY - JOUR
AU - Pourfarzib M
AU - Shekarchi M
AU - Rastegar H
AU - Akbariadergani B
AU - Mehramizi A
AU - Dinarvand R
TI - Molecularly Imprinted Nanoparticles Prepared by Miniemulsion Polymerization As a Sorbent for Selective Extraction and Purification of Efavirenz From Human Serum and Urine
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
VL - 974
IS -
SP - 1
EP - 8
PY - 2015
ER -

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