Rat Lung Decellularization Using Chemical Detergents for Lung Tissue Engineering Publisher Pubmed



Tebyanian H^{1, 2} ; Karami A^{1, 2} ; Motavallian E³ ; Samadikuchaksaraei A^{4, 5} ; Arjmand B⁶ ; Nourani MR¹ Show Affiliations
Source: Biotechnic and Histochemistry Published:2019

Abstract

Although pulmonary diseases account for a large number of deaths in the world, most have no treatment other than transplantation. New therapeutic methods for lung treatment include lung tissue engineering and regenerative medicine. Lung decellularization has been used to produce an appropriate scaffold for recellularization and implantation. We investigated 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS) with sodium dodecyl sulfate (SDS) and Triton X-100 detergents for effecting rat lung decellularization. We evaluated using conventional histology, immunofluorescence staining and SEM methods for removing nuclear material while leaving intact extracellular matrix proteins and three-dimensional architecture. We investigated different concentrations of CHAPS, SDS and Triton X-100 for different periods. We found that 2 mM CHAPS + 0/1% SDS for 48 h was the best among the treatments investigated. Our method can be used to produce an appropriate scaffold for recellularization by stem cells and for investigations ex vivo and in vivo. © 2018, © 2018 The Biological Stain Commission.