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Comparison of Engineered Cartilage Based on Bmscs and Chondrocytes Seeded on Pva-Ppu Scaffold in a Sheep Model Publisher Pubmed



Taghizadehjahed M1 ; Sepahdar A2 ; Rabiee N3, 4, 5 ; Nazbar A4 ; Farzadmohajeri S1, 6 ; Dehghan MM1, 6 ; Shokrgozar MA4 ; Majidi M4, 7 ; Mardjanmehr SH8 ; Aminianfar H6 ; Akbari Javar H9 ; Bonakdar S4
Authors

Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials Published:2022


Abstract

In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In addition, the H&E analysis and other related tests demonstrated the formation of neocartilage tissue in a sheep model. The results were found to be promising for cartilage tissue engineering and verified that the isolated chondrocyte cultures on the synthesized substrate retain their original composition. © 2022 Wiley Periodicals LLC.
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