Comparison of Engineered Cartilage Based on Bmscs and Chondrocytes Seeded on Pva-Ppu Scaffold in a Sheep Model Publisher Pubmed



Taghizadehjahed M¹ ; Sepahdar A² ; Rabiee N^{3, 4, 5} ; Nazbar A⁴ ; Farzadmohajeri S^{1, 6} ; Dehghan MM^{1, 6} ; Shokrgozar MA⁴ ; Majidi M^{4, 7} ; Mardjanmehr SH⁸ ; Aminianfar H⁶ ; Akbari Javar H⁹ ; Bonakdar S⁴ Show Affiliations
Source: Journal of Biomedical Materials Research - Part B Applied Biomaterials Published:2022

Abstract

In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In addition, the H&E analysis and other related tests demonstrated the formation of neocartilage tissue in a sheep model. The results were found to be promising for cartilage tissue engineering and verified that the isolated chondrocyte cultures on the synthesized substrate retain their original composition. © 2022 Wiley Periodicals LLC.