Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells

Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells Publisher

Ghasempour G^{1, 2} ; Zamanigarmsiri F³ ; Mohammadi A⁴ ; Najafi M¹

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1. Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
2. Student Research Committee, Iran University of medical sciences, Tehran, Iran
3. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Clinical Biochemistry, Faculty of medicine, Tarbiat Modares University, Tehran, Iran

Source: Gene Reports Published:2021

Abstract

Background and aims: Saturated fatty acids are known to involve in atherosclerosis through different biologic pathways. The aim of this study was to investigate the effects of palmitic acid (PA) on the tyrosine-protein kinase (HCK) gene and protein expression levels in vascular smooth muscle cells (VSMCs). Methods: The human Vascular Smooth Muscle Cells (VSMC) were treated with palmitic acid (0.5 mM, 24 h) based on the cellular viability studies. The HCK gene and protein expression levels were measured by real-time qRT-PCR and western blotting techniques, respectively. Oil Red O staining method was used to determine the intracellular lipid values. Results: The HCK gene expression level was increased significantly in the PA-treated VSMCs (p = 0.02). The total and phosphorylated HCK (p-HCK) protein expression levels increased in VSMCs. There was a significant increase in p-HCK value (P = 0.001). Conclusion: The results showed that the palmitic acid increases intracellular p-HCK value so that it may affect the HCK-mediated pathways in VSMCs. © 2021

Style	Citing Format
MLA	Ghasempour G, et al.. "Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells." Gene Reports, vol. 25, no. , 2021, pp. -.
APA	Ghasempour G, Zamanigarmsiri F, Mohammadi A, Najafi M (2021). Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells. Gene Reports, 25(), -.
Chicago	Ghasempour G, Zamanigarmsiri F, Mohammadi A, Najafi M. "Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells." Gene Reports 25, no. (2021): -.
Harvard	Ghasempour G et al. (2021) 'Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells', Gene Reports, 25(), pp. -.
Vancouver	Ghasempour G, Zamanigarmsiri F, Mohammadi A, Najafi M. Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells. Gene Reports. 2021;25():-.
BibTex	@article{ author = {Ghasempour G and Zamanigarmsiri F and Mohammadi A and Najafi M}, title = {Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells}, journal = {Gene Reports}, volume = {25}, number = {}, pages = {-}, year = {2021} }
RIS	TY - JOUR AU - Ghasempour G AU - Zamanigarmsiri F AU - Mohammadi A AU - Najafi M TI - Palmitic Acid Increases Hck Gene and Protein Expression Levels in Vascular Smooth Muscle Cells JO - Gene Reports VL - 25 IS - SP - EP - PY - 2021 ER -

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