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Expression of Adr-Α1, 2 and Adr-Β2 in Cumulus Cell Culture of Infertile Women With Polycystic Ovary Syndrome and Poor Responder Who Are a Candidate for Ivf: The Novel Strategic Role of Clonidine in This Expression Publisher Pubmed



Zangeneh FZ1 ; Bagheri M2 ; Shoushtari MS3 ; Naghizadeh MM4
Authors
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Authors Affiliations
  1. 1. Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Reproductive Health, Faculty of Nursing and Midwifery, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Chemical and Environment Department, Faculty of Engineering, UPM, Selangor, Malaysia
  4. 4. Nonproliferative Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran

Source: Journal of Receptors and Signal Transduction Published:2021


Abstract

Objective: Alpha and beta-adrenoceptors (ADR-α1, 2, and β2) play a regulatory role in the folliculogenesis and steroidogenesis in the ovarian follicles. This study aimed to measure these adrenoceptors mRNA and its protein levels in cumulus cells (CCs) culture of poor ovarian reserve (POR) and polycystic ovarian syndrome (PCOS) infertile women (IVF candidate) and the effect of clonidine treatment at CCs culture. Methods: This case/control study was conducted in 2017 includes a control (donation oocytes) and two studies (PCO and POR) groups. The ovulation induction drugs were prescribed in all groups. After the oocyte puncture, the follicular fluid was collected and CCs were isolated were cultured. RNA was extracted and cDNA was synthesized and designed the primer for the ADR-α1, 2 and ADR-β2 gene expression. The protein levels were investigated by Western Blot. Results: The results showed a high level of three adrenergic expressions in PCO women compared to the control group (p-value <.001), which can be reduced by clonidine. POR group showed a significant decrease in the gene expression of ADR-α1 (p-value =.004) and ADR-α2 (p-value =.003) compared to the control group and clonidine treatment had no effect. Conclusion: The significant increase of three adrenoceptors gene expression and protein levels in CCs culture indicate to the hyperactivity of the ovarian sympathetic nervous system at the receptor levels in women with PCOS, and clonidine confirmed it by reducing this expression. In POR women, the reduction of ADR-α1, 2 expressions maybe lead to the aging process in the ovary. © 2020 The Research Council of Tehran University of Medical Sciences.
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