Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies

Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies Publisher

Rahmanian M¹ ; Seyfoori A¹ ; Dehghan MM² ; Eini L¹ ; Naghib SM^{1, 3} ; Gholami H⁴ ; Farzad Mohajeri S⁴ ; Mamaghani KR³ ; Majidzadeha K¹

Show Affiliations

1. Biomaterials and Tissue Engineering Department, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran
2. Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
3. Nanotechnology Department, School of Advanced Technologies, Iran University of Science and Technology (IUST), P.O. Box 16846-13114, Tehran, Iran
4. Institute of Biomedical Research, University of Tehran, Tehran, Iran

Source: Journal of the Taiwan Institute of Chemical Engineers Published:2019

Abstract

Bone is known as the most prevalent, metastatic site of breast cancer; however, it's mechanism details are unknown, so developing a suitable scaffold for bone defect regeneration and sustain drug release after tumor resection can considerably inhibit the tumor recurrence after a while. Here, synthesized gelatin/beta-tricalcium phosphate (β-TCP) nanocomposite scaffold loaded with zoledronic acid (ZA) drug with different β-TCP concentrations, crosslinker and varying drug doses were investigated to highlight their effect on biological properties of the scaffold contained bone cells. The scanning electron microscopy (SEM) illustrated porous structure (50–200 µm) of gelatin reinforced with β-TCP spherical nanoparticles (around 90 nm diameter) that could be a proper matrix for bone cells proliferation. In vitro biological analysis by cytotoxicity assay (MTT test) as well as osteoblast cell line (G292) attachment test showed that the scaffolds were biocompatible and non-toxic. Also, higher β-TCP nanoparticle concentration enhanced the rate of cell proliferation on the scaffold. Further investigations indicated that the type of cell seeding into porous scaffolds should be considered as a significant parameter in cell behavior on the scaffold. The better cell attachment was observed in agarose gel wells in comparison with the petri dish one. Nanocomposite samples were loaded with ZA drug and its effects on the cell proliferation were investigated in vitro and in vivo as well. Histopathological results showed that the new bone formation was established more than 75% in the whole area of the defect after 3 and 4 months. For the first time, nanoscaled β-TCP/gelatin composite was proposed as a potential for controlled drug release and local resected tumor treatment. All findings besides in vitro and in vivo analyses, recommend gelatin/nano beta-tricalcium phosphate/drug scaffolds as a promising three-dimensional environment for repairing the resected bone tissue in primary or metastatic bone sites. © 2019 Taiwan Institute of Chemical Engineers

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Experts (# of related papers)

Reza Faridi Majidi (1)

Style	Citing Format
MLA	Rahmanian M, et al.. "Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies." Journal of the Taiwan Institute of Chemical Engineers, vol. 101, no. , 2019, pp. 214-220.
APA	Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, Farzad Mohajeri S, Mamaghani KR, Majidzadeha K (2019). Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies. Journal of the Taiwan Institute of Chemical Engineers, 101(), 214-220.
Chicago	Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, Farzad Mohajeri S, Mamaghani KR, Majidzadeha K. "Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies." Journal of the Taiwan Institute of Chemical Engineers 101, no. (2019): 214-220.
Harvard	Rahmanian M et al. (2019) 'Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies', Journal of the Taiwan Institute of Chemical Engineers, 101(), pp. 214-220.
Vancouver	Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, et al.. Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies. Journal of the Taiwan Institute of Chemical Engineers. 2019;101():214-220.
BibTex	@article{ author = {Rahmanian M and Seyfoori A and Dehghan MM and Eini L and Naghib SM and Gholami H and Farzad Mohajeri S and Mamaghani KR and Majidzadeha K}, title = {Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies}, journal = {Journal of the Taiwan Institute of Chemical Engineers}, volume = {101}, number = {}, pages = {214-220}, year = {2019} }
RIS	TY - JOUR AU - Rahmanian M AU - Seyfoori A AU - Dehghan MM AU - Eini L AU - Naghib SM AU - Gholami H AU - Farzad Mohajeri S AU - Mamaghani KR AU - Majidzadeha K TI - Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies JO - Journal of the Taiwan Institute of Chemical Engineers VL - 101 IS - SP - 214 EP - 220 PY - 2019 ER -

Style

Citing Format

MLA

Rahmanian M, et al.. "Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies." Journal of the Taiwan Institute of Chemical Engineers, vol. 101, no. , 2019, pp. 214-220.

APA

Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, Farzad Mohajeri S, Mamaghani KR, Majidzadeha K (2019). Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies. Journal of the Taiwan Institute of Chemical Engineers, 101(), 214-220.

Chicago

Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, Farzad Mohajeri S, Mamaghani KR, Majidzadeha K. "Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies." Journal of the Taiwan Institute of Chemical Engineers 101, no. (2019): 214-220.

Harvard

Rahmanian M et al. (2019) 'Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies', Journal of the Taiwan Institute of Chemical Engineers, 101(), pp. 214-220.

Vancouver

Rahmanian M, Seyfoori A, Dehghan MM, Eini L, Naghib SM, Gholami H, et al.. Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies. Journal of the Taiwan Institute of Chemical Engineers. 2019;101():214-220.

BibTex

@article{ author = {Rahmanian M and Seyfoori A and Dehghan MM and Eini L and Naghib SM and Gholami H and Farzad Mohajeri S and Mamaghani KR and Majidzadeha K}, title = {Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies}, journal = {Journal of the Taiwan Institute of Chemical Engineers}, volume = {101}, number = {}, pages = {214-220}, year = {2019} }

RIS

TY - JOUR
AU - Rahmanian M
AU - Seyfoori A
AU - Dehghan MM
AU - Eini L
AU - Naghib SM
AU - Gholami H
AU - Farzad Mohajeri S
AU - Mamaghani KR
AU - Majidzadeha K
TI - Multifunctional Gelatin–Tricalcium Phosphate Porous Nanocomposite Scaffolds for Tissue Engineering and Local Drug Delivery: In Vitro and in Vivo Studies
JO - Journal of the Taiwan Institute of Chemical Engineers
VL - 101
IS -
SP - 214
EP - 220
PY - 2019
ER -

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