The Correlation of Serum S100β Protein Levels and Hippocampal Seladin-1 Gene Expression in a Rat Model of Sporadic Alzheimer’S Disease



Hosseinzadeh S^{1, 2} ; Zahmatkesh M¹ ; Hassanzadeh GR^{1, 3} ; Karimian M^{1, 4} ; Heidari M⁵ ; Karami M⁶ Show Affiliations
Source: Tehran University Medical Journal Published:2015

Abstract

Background: Seladin-1 protein protects the neural cells against amyloid beta toxicity and its expression decreased in vulnerable regions of Alzheimer’s disease (AD) brains. On the other hand, changes in serum levels of S100β have been considered as a marker of brain damage in neurodegenerative diseases. Furthermore, this study was carried out to determine the relation between the change profile of serum S100β protein levels and hippocampal Seladin-1 gene expression in a rat model of sporadic AD. Methods: In this experimental study that established in Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Science, from March 2011 to April 2013, 72 animals were randomly divided into control, 4, 7, 14, and 21days ICV-STZ/Saline administrated rats. Alzheimer’s model was induced by intracerebroventricular (ICV) injections of streptozotocin (STZ) [3 mg/kg] on days 1 and 3. Serum levels of S100β and hippocampal Seladin-1 gene expression were evaluated in experimental groups. The initial and step-through latencies (STL) were determined using passive avoidance test. Results: Serum levels of S100β were significantly different between the STZ-7 day and STZ-14 day groups in comparison with the control, saline and STZ-4 day groups. As well as, there was a significant difference between the STZ-7 day group in comparison with the STZ-14 day and STZ-21 day groups (P=0.0001). Hippocampal Seladin-1 gene expression in STZ-14 day and STZ-21 day groups significantly decreased as compared to the control, saline and STZ-4 day groups (P=0.0001). However, significant correlation was detected between serum S100β protein decrement and Seladin-1 down regulation (P=0.001). Also, the STL was significantly decreased in 21 days ICV-STZ administrated rats as compared to the control or saline groups (P=0.001). Conclusion: Monitoring the changes of serum S100β protein levels by relationship with changes in hippocampal Seladin-1 gene expression can be a useful indicator of neuronal damage in patients with Alzheimer’s disease. © 2015, Tehran University of Medical Sciences. All rights reserved.