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Effects of Oleoylethanolamide Supplementation on the Expression of Lipid Metabolism-Related Genes and Serum Nrg4 Levels in Patients With Non-Alcoholic Fatty Liver Disease: A Randomized Controlled Trial Publisher Pubmed



Tutunchi H1 ; Ebrahimimameghani M2 ; Hosseinzadehattar MJ3 ; Roshanravan N4 ; Mobasseri M1 ; Najafipour F1 ; Naeini F3 ; Naghshi S5 ; Asghari S6 ; Akbarzadeh M6 ; Soleimanzadeh H7 ; Ostadrahimi A8
Authors
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Authors Affiliations
  1. 1. Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  2. 2. Nutrition Research Center, Department of Biochemistry and Diet Therapy, Faculty of Nutrition & Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
  3. 3. Department of Clinical Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Cardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
  6. 6. Stem Cell and Regenerative Medicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
  7. 7. Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
  8. 8. Nutrition Research Center, Department of Clinical Nutrition, School of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Clinical Nutrition ESPEN Published:2023


Abstract

Background: This study investigated the effects of oleoylethanolamide (OEA) supplementation on the expression levels of SIRT1, AMPK, PGC-1α, PPAR-γ, CEBP-α and CEBP-β genes and serum neuregulin 4 (NRG4) levels in patients with non-alcoholic fatty liver diseases (NAFLD). Methods: Sixty obese patients with NAFLD were equally allocated into either OEA or placebo group for 12 weeks. The mRNA expression levels of genes were determined using the reverse transcription polymerase chain reaction (RT-PCR) technique. Serum NRG4 level was also assessed using an enzyme-linked immunosorbent assay (ELISA) kit. Results: At the endpoint, mRNA expression levels of SIRT1(p = 0.001), PGC-1α (p = 0.011) and AMPK (p = 0.019) were significantly higher in the OEA group compared to placebo group. However, no significant differences were observed in the expression levels of PPAR-γ, CEBP-α and CEBP-β between the two groups. Serum NRG4 levels significantly increased in the OEA group compared with the placebo group after controlling for confounders (p = 0.027). In the OEA group, significant relationships were found between percent of changes in the expression levels of the SIRT1, AMPK and PGC-1α as well as serum NRG4 level with percent of changes in some anthropometric measures. Moreover, in the intervention group, percent of changes in high-density lipoprotein cholesterol was positively correlated with percent of changes in the expression levels of the SIRT1 and AMPK. While, percent of changes in triglyceride was inversely correlated with percent of changes in the expression levels of SIRT1. Conclusion: OEA could beneficially affect expression levels of some lipid metabolism-related genes and serum NRG4 level. “Registered under Iranian Registry of Clinical Trials Identifier no: IRCT20090609002017N32”. © 2023
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