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Synthesis of a Copolymer Carrier for Anticancer Drug Luteolin for Targeting Human Breast Cancer Cells



Maleki M1 ; Aidy A1 ; Karimi E1, 2 ; Shahbazi S3 ; Safarian N4 ; Abbasi N1, 5
Authors
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Authors Affiliations
  1. 1. Biotechnology and Medicinal Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran
  2. 2. Department of Chemistry, Ilam Branch, Islamic Azad University, Ilam, Iran
  3. 3. Department of Internal Medicine, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
  4. 4. Department of Medical Oncology and Hematology, Faculty of Medicine, Tehran University of Medical Science, Tehran, Iran
  5. 5. Department of Pharmacology & Biotechnology and Medicinal Plants Research Center, Medical School, Ilam University of Medical Sciences, Ilam, Iran

Source: Journal of Traditional Chinese Medicine Published:2019

Abstract

OBJECTIVE: To focus a new chemoprevention approach that uses nanotechnology to deliver luteolin to human breast cancer cells (MCF-7), and its underlying mechanism. METHODS: Water-soluble copolymer-encapsulated nanoparticle-luteolin (CENL) was formulated using the hydrophobic drug luteolin. The ability to load and release he anticancer drug into/from the synthesized hyperbranched polyester was evaluated by high-performance liquid chromatography. The successful synthesis of CENL was supported by analytical techniques including fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, gel permeation chromatography, and dynamic light scattering. Cell viability was measured using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide color method. Reactive oxygen species (ROS) were measured using a dichlorofluorescein probe and intracellular calcium (Cai2+) was evaluated with a flu3-AM probe. RESULTS: The results showed that the drug delivery system is stable and that the loading capacity is high. Treatment with nanoparticles containing luteolin and free luteolin resulted in cell death in breast cancer cells at high concentrations [IC50 (33 ± 4) and (48 ± 6) μM, respectively]. At high concentrations, CENL reduced cell viability and increased ROS and Cai2+ production. CONCLUSION: Our results demonstrate that CENL has potential for human breast cancer treatment. © 2019 JTCM. All rights reserved.
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