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Simultaneous Dual-Wavelength Laser Irradiation Against Implant-Adherent Biofilms of Staphylococcus Aureus, Escherichia Coli, and Candida Albicans for Improved Antimicrobial Photodynamic Therapy Publisher



Afrasiabi S1 ; Benedicenti S2 ; Signore A3 ; Arshad M4, 5 ; Chiniforush N2
Authors
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Authors Affiliations
  1. 1. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, 1441987566, Iran
  2. 2. Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV, 6, Genoa, 16132, Italy
  3. 3. Therapeutic Dentistry Department, Institute of Dentistry, I.M. Sechenov First Moscow State Medical University, Trubetskaya Str. 8, b. 2, Moscow, 119992, Russian Federation
  4. 4. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, 1441987566, Iran
  5. 5. Department of Prosthodontics, School of Dentistry, Tehran University of Medical Sciences, International Campus, Tehran, 1441987566, Iran

Source: Bioengineering Published:2024


Abstract

The efficiency of antimicrobial photodynamic therapy (PDT) might be improved by using multiple wavelengths. This study investigates the sensitivity of implant-adherent biofilms of Staphylococcus aureus, Escherichia coli, and Candida albicans to indocyanine green (ICG)-808 nm diode laser, toluidine blue O (TBO)-635 nm diode laser, and hydrogen peroxide (HP)-980 nm diode laser and their combination when irradiated with dual-wavelength laser irradiation (simultaneously 980–635 nm or 980–808 nm). After an incubation period of 72 h, the infected implants were randomly divided into seven different treatment modalities: Control, HP, HP-PDT, TBO-PDT, HP-TBO-PDT, ICG-PDT, and HP-ICG-PDT. After the treatments, the colony-forming units (CFUs)/mL and reactive oxygen species (ROS) generation were determined. All evaluated disinfection methods were significantly effective against the three investigated bacteria compared to the control. The combined treatment of HP-ICG-PDT or HP-TBO-PDT had the greatest antibacterial effect compared to each treatment alone. There were statistical differences between HP-ICG-PDT and ICG-PDT or HP-TBO-PDT and TBO-PDT for all three bacteria studied. PDT with simultaneous dual-wavelength laser irradiation is an efficient strategy to improve the therapeutic effect of PDT. © 2024 by the authors.
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