Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Riboflavin, Nanocurcumin, and Hydrogen Peroxide Under Light Conditions: Reduction of Mature Dental Biofilms and Enamel Mineral Loss Publisher Pubmed



Afrasiabi S1 ; Al Gburi AQK2 ; Ranjbar Omrani L2 ; Chiniforush N3 ; Moradi Z2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2024


Abstract

Background: Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of Streptococcus mutans and Lactobacillus acidophilus dual-species biofilms and their effect on the enamel mineral loss. Materials and methods: The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (n = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Results: While HP-PDT showed a reduction of 37% (p < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, p < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups. Conclusions: The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria. © 2024
Related Docs
View other Related Docs
1. Simultaneous Dual-Wavelength Laser Irradiation Against Implant-Adherent Biofilms of Staphylococcus Aureus, Escherichia Coli, and Candida Albicans for Improved Antimicrobial Photodynamic Therapy, Bioengineering (2024)
2. An in Vitro Study on the Efficacy of Hydrogen Peroxide Mediated High-Power Photodynamic Therapy Affecting Enterococcus Faecalis Biofilm Formation and Dispersal, Photodiagnosis and Photodynamic Therapy (2023)
3. Destruction of Cariogenic Biofilms by Antimicrobial Photodynamic Therapy Mediated by Phycocyanin and Toluidine Blue Along With Sodium Fluoride Varnish or Titanium Tetrafluoride, Photodiagnosis and Photodynamic Therapy (2024)
4. The Effect of Different Output Powers of Blue Diode Laser Along With Curcumin and Riboflavin Against Streptococcus Mutans Around Orthodontic Brackets: An in Vitro Study, Biomedicines (2023)
5. Synergistic Antimicrobial Effect of Photodynamic Inactivation and Sweeps in Combined Treatment Against Enterococcus Faecalis in a Root Canal Biofilm Model: An in Vitro Study, Applied Sciences (Switzerland) (2023)
6. Sweeps-Assisted Antibacterial Photodynamic Therapy Against Dual-Species Biofilms in Mandibular Molars: An in Vitro Study, Pharmaceuticals (2025)
7. Quorum Quenching of Streptococcus Mutans Via the Nano-Quercetin-Based Antimicrobial Photodynamic Therapy As a Potential Target for Cariogenic Biofilm, BMC Microbiology (2022)
8. Anti-Biofilm and Anti-Metabolic Effects of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture Against Streptococcus Mutans in Experimental Biofilm Caries Model on Enamel Slabs, Photodiagnosis and Photodynamic Therapy (2020)
Experts (# of related papers)
View all Related Experts
Maryam Pourhajibagher (8)
Abbas Bahador (7)
Other Related Docs
9. In Vitro Antibiofilm Effect of Different Irradiation Doses in Infected Root Canal Model, Photodiagnosis and Photodynamic Therapy (2024)
10. The Potential Application of Natural Photosensitizers Used in Antimicrobial Photodynamic Therapy Against Oral Infections, Pharmaceuticals (2022)
11. In Vitro Antibacterial Activity of Photoactivated Flavonoid Glycosides Against Acinetobacter Baumannii, AMB Express (2024)
12. Effect of Antimicrobial Photodynamic Therapy on the Counts of Salivary Streptococcus Mutans in Children With Severe Early Childhood Caries, Photodiagnosis and Photodynamic Therapy (2017)
13. Postbiotic Mediators Derived From Lactobacillus Species Enhance Riboflavin-Mediated Antimicrobial Photodynamic Therapy for Eradication of Streptococcus Mutans Planktonic and Biofilm Growth, BMC Oral Health (2024)
14. Evaluation of the Photodynamic Therapy With Riboflavin and Curcumin on Shear Bond Strength of Orthodontic Bracket: An in Vitro Study, Photodiagnosis and Photodynamic Therapy (2022)
15. The Influence of Different Mode of Power Density During Antimicrobial Photodynamic Therapy for Photokilling of Streptococcus Mutans, Photodiagnosis and Photodynamic Therapy (2023)
16. Theranostic Nanoplatforms of Emodin-Chitosan With Blue Laser Light on Enhancing the Anti-Biofilm Activity of Photodynamic Therapy Against Streptococcus Mutans Biofilms on the Enamel Surface, BMC Microbiology (2022)
17. Analysis of Glucosyltransferase Gene Expression of Clinical Isolates of Streptococcus Mutans Obtained From Dental Plaques in Response to Sub-Lethal Doses of Photoactivated Disinfection, Photodiagnosis and Photodynamic Therapy (2018)
18. The Effect of Apdt With Natural and Chemical Photosensitizers on Reduction of Bacteria Around Orthodontic Brackets, Lasers in Medical Science (2025)
19. Antimicrobial Action of Photodynamic Therapy on Enterococcus Faecalis Biofilm Using Curing Light, Curcumin and Riboflavin, Australian Endodontic Journal (2022)
20. New Strategies to Enhance Antimicrobial Photo-Sonodynamic Therapy Based on Nanosensitizers Against Bacterial Infections, Folia Microbiologica (2025)
21. Reduction of Multispecies Biofilms on an Acrylic Denture Base Model by Antimicrobial Photodynamic Therapy Mediated by Natural Photosensitizers, Pharmaceuticals (2024)
22. The Evaluation of Sweeps Plus Antimicrobial Photodynamic Therapy With Indocyanine Green in Eliminating Enterococcus Faecalis Biofilm From Infected Root Canals: An in Vitro Study, Biomedicines (2023)
23. Effect of Nanomicelle Curcumin-Based Photodynamic Therapy on the Dynamics of White Spot Lesions and Virulence of Streptococcus Mutans in Patients Undergoing Fixed Orthodontic Treatment: A Randomized Double-Blind Clinical Trial, Photodiagnosis and Photodynamic Therapy (2022)
24. Nanostructures As Targeted Therapeutics for Combating Oral Bacterial Diseases, Biomedicines (2021)