Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Evaluation of Riboflavin, Nanocurcumin, and Hydrogen Peroxide Under Light Conditions: Reduction of Mature Dental Biofilms and Enamel Mineral Loss Publisher Pubmed



Afrasiabi S1 ; Al Gburi AQK2 ; Ranjbar Omrani L2 ; Chiniforush N3 ; Moradi Z2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Restorative Dentistry, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2024


Abstract

Background: Biofilms are a potential harbor for many microorganisms. The aim of this study was to test the efficacy of riboflavin (Rib), nano-micelle curcumin (NC), and hydrogen peroxide (HP), alone and in combination with the respective light (light-emitting diode (LED) or 980 nm diode laser) on the reduction of Streptococcus mutans and Lactobacillus acidophilus dual-species biofilms and their effect on the enamel mineral loss. Materials and methods: The biofilms were formed on saliva-coated enamel slabs. Then, the biofilms were treated with antimicrobial photodynamic therapy (PDT) based on LED, Rib, and NC photosensitizers and with HP also based on a 980 nm diode laser (n = 8 per group). A crystal violet assay was performed to determine the reduction of the dual-species biofilms. The enamel slabs were analyzed for calcium and phosphorus content by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX). Results: While HP-PDT showed a reduction of 37% (p < 0.001), PDT with NC resulted in an even greater reduction of dual-species biofilms (40%, p < 0.001) than HP- and Rib-mediated PDT. In the EDX test, no significant difference was found between the control group and the treatment groups. Conclusions: The use of natural photosensitizers such as NC in PDT has an effect that may be potentially important in reducing caries-causing bacteria. © 2024
Experts (# of related papers)
Other Related Docs