Antibacterial/Antifungal Activity of Extracted Chitosan From American Cockroach (Dictyoptera: Blattidae) and German Cockroach (Blattodea: Blattellidae) Publisher Pubmed



Basseri H¹ ; Bakhtiyari R² ; Hashemi SJ³ ; Baniardelani M¹ ; Shahraki H¹ ; Hosainpour L³ Show Affiliations
Source: Journal of Medical Entomology Published:2019

Abstract

We investigated bactericidal and fungicidal properties of chitosan extracted from adults and nymphs from both German cockroach, Blattella germanica (Blattodea: Blattellidae) and American cockroach, Periplaneta americana (Dictyoptera: Blattidae).The cuticle of adults and nymphs extracted from both cockroaches were dried and ground. The powders were demineralized and deproteinized followed by deacetylation using NaOH. Finally, the chitosan yields were examined for antibacterial and antifungal activities.The degree of deacetylation (DD) was different between adults and nymph stages.The antimicrobial effect of American cockroach chitosan (ACC) and German cockroach chitosan (GCC) was tested against four bacteria and four fungi.The extracted chitosans from American cockroach, Periplaneta americana and German Cockroach, Blattella germanica suppressed the growth of Gram-negative/positive bacteria except Micrococcus luteus. The growth of Aspergillus flavus and Aspergillus Niger were notability inhibited by the extracted chitosans.The antimicrobial effect of the chitosan depended on the cockroach species, with chitosan of the American cockroach showing more inhibitory effect. This difference may be due to differences in the structure of chitin between the two cockroach species. © The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.