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Coordination of Las Regulated Virulence Factors With Multidrug-Resistant and Extensively Drug-Resistant in Superbug Strains of P. Aeruginosa Publisher Pubmed



Dehbashi S1 ; Pourmand MR2 ; Alikhani MY1 ; Asl SS3 ; Arabestani MR1, 4
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  2. 2. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Anatomy, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  4. 4. Nutrition Health Research Center, Hamadan University of Medical Sciences, Hamadan, Iran

Source: Molecular Biology Reports Published:2020


Abstract

Successful pathogenicity often resulted from a complicated association between virulence and antibiotic resistance in Pseudomonas aeruginosa infections. Therefore, the current study aimed to investigate the relationship between the las system and antibiotic resistance. Seventy-three (73) P. aeruginosa isolates were collected from burn wounds (26.02%), blood cultures (30.13%), catheters (12.32%), and urine culture (31.50%). Among the 73 collected isolates, 22 isolates were considered as multi-drug resistant (MDR) and 11 isolates as extensively-drug resistant (XDR). Furthermore, phenazines and LasA protease were detected among 21.91% and 32.87% of isolates, respectively. Quantitative real-time PCR assessment of KPC, MBL, and lasI/R indicated that resistance and virulence factors are more expressed in XDR strains than MDR strains. Also, the expression level of KPC and MBL reduced in non-biofilm forming strains. However, increased expression levels of lasI, lasR, and the KPC genes were observed in LasA and LasB protease producing strains. Interestingly, 16 known sequence types (including ST108, ST260, ST217) and three novel STs (ST2452, ST2427, and ST2542) were characterized among the collected isolates, which are related to the virulence and resistance. In MDR-XDR strains, a strong correlation between lasI/R and the variants of antibiotic resistance genes was found. In conclusion, the pathogenicity of P. aeruginosa may increase the prevalence of antibiotic-resistant strains. © 2020, Springer Nature B.V.
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