Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Comparison of Cytotoxicity Between Mineral Trioxide Aggregate Mixed With Chlorhexidine and Common Endodontic Regeneration Medicaments on Periodontal Ligament Stem Cells: An in Vitro Study Publisher



Ghabraei S1 ; Afkhami F1 ; Shamshiri AR2 ; Mohammadi Z1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Research Center for Caries Prevention, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Frontiers in Dentistry Published:2023


Abstract

Objectives: The combination of mineral trioxide aggregate (MTA) and 2% chlorhexidine (CHX) has been recently introduced as an intracanal medicament. The aim of this study was to evaluate the potential cytotoxic effects of MTA mixed with 2% chlorhexidine gel on human periodontal ligament stem cells (PDLSCs) and compare it with other common endodontic regeneration medicaments. Materials and Methods: Minimum inhibitory concentration and minimum bactericidal concentration of six experimental groups against Enterococcus faecalis was determined. The study groups consisted of RetoMTA mixed with 2% chlorhexidine gel (MTA+CHX), calcium hydroxide (CH), CH mixed with CHX gel, two concentrations of double antibiotic paste, and 2% CHX. The direct cytotoxic effect of minimum bactericidal concentration was evaluated by MTT on PDLSCs on days 1, 3, and 7. One-way ANOVA and post hoc tests were used for data analysis (P<0.05). Results: The viability of cells treated with MTA+CHX decreased significantly over time (P<0.05) making this group the most cytotoxic intracanal medicament on the 3rd and 7th days of treatment. On day one, the highest viability percentage was detected in the CH+CHX group followed by the CHX group. On day 3, CH+CHX and CHX groups displayed the highest viability percentage. On day 7, the highest viability was observed in the CHX group, which showed no significant difference with the control group (P=0.12). Conclusion: Regarding the antimicrobial potency of intracanal medicaments at minimum bactericidal concentration levels, CHX gel appears to be the least cytotoxic drug, while MTA+CHX shows the highest reduction in viability percentage. © 2023, Tehran University of Medical Sciences. All rights reserved.
Related Docs
1. Unpredictable Outcomes of a Regenerative Endodontic Treatment, Case Reports in Dentistry (2021)
2. Cytotoxicity Evaluation of Minimum Antibacterial Values of Different Medicaments Used in Endodontic Regenerative Procedures, European Journal of Dentistry (2019)
3. Antibiofilm Efficacy of Silver Nanoparticles As a Vehicle for Calcium Hydroxide Medicament Against Enterococcus Faecalis, Journal of Dentistry (2015)
4. Prevention of Tooth Discoloration Due to Calcium-Silicate Cements: A Review, Dental Hypotheses (2019)
5. Second-Generation Platelet Concentrate (Platelet-Rich Fibrin) As a Scaffold in Regenerative Endodontics: A Case Series, Journal of Endodontics (2017)
6. Regenerative Property of Prf Used As Capping Material in Pulpotomy in Dogs, Biomedical Research (India) (2017)
Experts (# of related papers)
View all Related Experts
Mehrfam Khoshkhounejad (2)
Behnam Bolhari (2)