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Influence of Temperature on the Antibacterial Activity of Sodium Hypochlorite Publisher Pubmed



Giardino L1 ; Mohammadi Z2 ; Beltrami R3 ; Poggio C4 ; Estrela C5 ; Generali L6
Authors
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Authors Affiliations
  1. 1. Crotone, Italy
  2. 2. Iranian Center for Endodontic Research (ICER), Research Institute of Dental Sciences Shahid Beheshti, University of Medical Sciences, Iranian National Elite Foundation, Tehran, Iran
  3. 3. Department of Brain, Behavioral Sciences, Section of Statistics, University of Pavia, Italy
  4. 4. Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, Section of Dentistry, University of Pavia, Pavia, Italy
  5. 5. Department of Stomatological Sciences, Dental School, UFG - Universidade Federal de Goias, Goiania, GO, Brazil
  6. 6. Department of Surgery, Medicine, Dentistry and Morphological Sciences with Transplant Surgery, Oncology and Regenerative Medicine Relevance, School of Dentistry, Endodontic Section, University of Modena and Reggio Emilia, Modena, Italy

Source: Brazilian Dental Journal Published:2016


Abstract

The aim of this study was to compare the antimicrobial activity of 5.25% NaOCl, Hypoclean and Chlor-Xtra at 20 °C and 45 °C in bovine root dentin. One-hundred-and-seventy dentin tubes prepared from bovine maxillary incisors were infected for 21 days with Enterococcus faecalis. The specimens were divided into the following groups: 1. 5.25% NaOCl 20 °C; 2. Hypoclean 20 °C; 3. Chlor-Xtra 20 °C; 4. 5.25% % NaOCl 45 °C; 5. Hypoclean 45 °C; 6. Chlor-Xtra 45 °C; 7. positive control; 8. negative control. Dentin chips were collected with round burs into Brain Heart Infusion (BHI) broth. After culturing, the number of colony-forming units (CFU) was counted. Statistical analyses were performed using descriptive statistics (mean, standard deviation, median), Shapiro-Wilk test, ANOVA and Tukey test. Significance level was set at p<0.05. In all experimental groups, CFU was minimum after treatment (day 0) and the obtained results were significantly different from each other at any period (p<0.05). After treatment, the Hypoclean and Chlor-Xtra showed the lowest numbers of CFU at 20 °C and 45 °C, whereas 5.25% NaOCl showed the highest number of CFU at both temperatures. In each group, the number of CFUs increased significantly with time (p<0.05). The antibacterial activity of Hypoclean and Chlor-Xtra at 45 °C were significantly greater than other tested solutions. © 2016, Associacao Brasileira de Divulgacao Cientifica. All rights reserved.
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