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In Vitro Antifungal Susceptibility Profiles of Candida Albicans Complex Isolated From Patients With Respiratory Infections Pubmed



Sharifynia S1 ; Badali H2 ; Sorkherizi MS1 ; Shidfar MR1 ; Hadian A1 ; Shahrokhi S3 ; Ghandchi G4 ; Rezaie S1
Authors
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Authors Affiliations
  1. 1. Department of Medical Mycology and Parasitology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Mycology and Parasitology, Pharmaceutical Sciences Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Islamic Azad University, Varamin-Pishva Branch, Varamin, Iran
  4. 4. Pediatric Infections Research Center, Shahid Beheshti University of Medical Science, Tehran, Iran

Source: Acta Medica Iranica Published:2016


Abstract

Candidiasis, the main opportunistic fungal infection has been increased over the past decades. This study aimed to characterize C.albicans species complex (C.albicans, C.dubliniensis, and C.africana) isolated from patients with respiratory infections by molecular tools and in vitro antifungal susceptibilities by using broth microdilution method according to CLSI M27-A3 guidelines. Totally, 121 respiratory samples were collected from patients with respiratory infections. Of these, 83 strains were germ tube positive and green colonies on chromogenic media, so initially identified as C.albicans species complex and subsequently were classified as C.albicans (89.15%), C.dubliniensis (9.63%), and C.africana (1.2%) based on PCR-RFLP and amplification of hwp1 gene. Minimum inhibitory concentration (MICs) results showed that all tested isolates of C.albicans complex were highly susceptible to triazole drugs. However, caspofungin had highest activity against C.albicans, C.dubliniensis, and C.africana. Our findings indicated the variety of antifungal resistance of Candida strains in different areas. These results may increase the knowledge about the local distribution of the mentioned strains as well as their antifungal susceptibility pattern which play an important role in appropriate therapy. © 2016 Tehran University of Medical Sciences. All rights reserved.
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