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The Effect of Lactobacillus Casei Consumption in Improvement of Obsessive–Compulsive Disorder: An Animal Study Publisher Pubmed



Sanikhani NS1 ; Modarressi MH2 ; Jafari P3 ; Vousooghi N4, 5 ; Shafei S6 ; Akbariqomi M1 ; Heidari R1 ; Lavasani PS1 ; Yazarlou F2 ; Motevaseli E1 ; Ghafourifard S7
Authors
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Authors Affiliations
  1. 1. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Microbiology Department, Science faculty, Islamic Azad University, Arak branch, Arak, Iran
  4. 4. Department of Neuroscience and Addiction Studies, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Research Center for Cognitive and Behavioral Sciences, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Molecular Medicine, School of Advanced Technologies in Medicine, International Campus Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Probiotics and Antimicrobial Proteins Published:2020


Abstract

Obsessive–compulsive disorder (OCD) is an important neuropsychiatric disorder worldwide. Common treatments of OCD include serotonergic antidepressants, which can cause potentially serious side effects. We assessed the effects of Lactobacillus casei (L. casei) Shirota consumption in an animal model of OCD. OCD-like symptoms were induced in rats by the chronic injection of the D2/D3 dopamine agonist quinpirole hydrochloride. Rats were classified into five groups of 6 rats. Four groups were injected chronically with quinpirole (0.5 mg/kg, twice weekly for 5 weeks). They were fed with L. casei Shirota (109 CF/g, daily for 4 weeks) (group 1), fluoxetine (10 mg/kg, daily for 4 weeks) (group 2), combination of L. casei Shirota and fluoxetine (group 3), and normal saline (positive control group). The last group did not receive dopamine agonist and was only injected with saline (negative control group). Expression levels of brain-derived neurotrophic factor (Bdnf), solute carrier family 6 member 4 (Slc6a4), and 5-hydroxytryptamine receptor type 2A (Htr2a) were assessed in orbitofrontal cortex tissues of all rats. Behavioral tests showed improvement of OCD signs in rats treated with L. casei Shirota, fluoxetine, and a combination of drugs. Quantitative PCR analysis showed a remarkable decrease in the expression of Bdnf and an increase in the expression of Htr2a in quinpirole-treated rats. After treatment with L. casei Shirota and fluoxetine, the expression level of Bdnf was increased remarkably, whereas Htr2a expression was decreased. The current study showed the effectiveness of L. casei Shirota in the treatment of OCD in a rat model. The beneficial effects of this probiotic are possibly exerted through the modulation of serotonin-related genes expression. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
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