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Evaluation of P21waf Expression and Cdkn1a Exon 2 Mutation in Salivary Adenoid Cystic Carcinoma Publisher



Taghavi N1 ; Yadegari Z1 ; Yazdani F2 ; Mashhadiabbas F1 ; Baghban AA3 ; Vafadar S4
Authors
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Authors Affiliations
  1. 1. Departments of Dental Biomaterials, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Pathology Department, Amir Alam Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Proteomics Research Center, Department of Basis Science, School of Rehabilitation, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Oral and Maxillofacial Pathology Department, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: World Cancer Research Journal Published:2019


Abstract

Objective: The P21waf is a tumor-suppressor protein encoded by CDKN1A gene. In this study, we evaluated P21waf expression and CDKN1A exon 2 mutation and their relationships with clinicopathological parameters and cancer development in salivary adenoid cystic carcinoma (ACC). Patient and Methods: Forty paraffin blocks from patients with salivary ACC were collected. Immunohistochemical staining was performed using P21 antibody. Genomic DNAs were extracted from the deparaffinized sections of the embedded tissue. Exon 2 of CDKN1A gene was amplified by PCR and the PCR products were sequenced. Spearman’s correlation coefficient, Fisher’s exact test, and Kruskal-Wallis test were used for data analysis. Results: A significant inverse correlation was observed between P21 expression and histologic grade (p=0.033, r=-0.338). The correlation of tumor size with recurrence (p=0.048) and tumor stage (p=0.046) was also evidenced. No mutation was detected in the exon 2 of CDKN1A gene. Conclusions: Regarding the association of P21 expression and histologic grade as a major prognostic indicator of ACC, P21 may be a useful prognostic indicator in ACC. On the other hand, CD-KN1A exon 2 mutation seems inapplicable as a risk factor for ACC development. © 2019, Verduci International. All rights reserved.