Effects of Two Isocalorie Diets With Different Amounts of Fat During Pregnancy and Lactation of C57bl/6 Mice on Gene and Protein Level of Sirtuin 1 in the Liver Tissue of Adult Offspring



Mousavi SN^{1, 2} ; Gharacheh S³ ; Dorraji MSS⁴ ; Hosseini E¹ ; Koohdani F³ Show Affiliations
Source: Iranian Journal of Diabetes and Metabolism Published:2023

Abstract

Background: Animal studies have shown maternal low/ high-calorie diet during pregnancy can alter metabolism of offspring through change in Sirtuin 1 (SIRT1) expression, as a metabolic sensor. However, there is no study on the effects of isocalorie diet. Herein, effects of two isocalorie diets with different amounts of fat were assessed on SIRT1 gene and protein level in the liver of male and female offspring. Methods: The control group received AIN93G diet. In this diet, 16% and 64% of total calorie were prepared from fat and carbohydrate, respectively. The intervention group received high fat AIN93G diet contained 48% and 32% of calorie from fat and carbohydrate, respectively. In both diets, fat was prepared from soy oil. Diets were isocaloric and 20% of total calorie was provided from protein. Mothers categorized to one of these diets and offspring received the control diet after weaning (3 weeks after the birth). Results: SIRT1 gene and protein levels were lower in male and female offspring born from mothers received high-fat diet than the controls (p<0.001). Conclusion: Change in maternal dietary fat, without increase in calorie, effects on gene and protein levels of SIRT-1 in the liver of next generation. © 2023 Iranian Journal of Diabetes and Metabolism and Tehran University of Medical Sciences. Published by Tehran University of Medical Sciences.