Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer

Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer Publisher Pubmed

Salehipour P¹ ; Nematzadeh M² ; Mobasheri MB^{1, 3} ; Afsharpad M³ ; Mansouri K⁴ ; Modarressi MH^{1, 3}

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1. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Genetic, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
3. Cancer Research Center, Cancer Institute of IRAN, Tehran University of Medical Sciences, Tehran, Iran
4. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Source: Biochimica et Biophysica Acta - Gene Regulatory Mechanisms Published:2017

Abstract

Testis specific gene antigen 10 (TSGA10) is a cancer testis antigen involved in the process of spermatogenesis. TSGA10 could also play an important role in the inhibition of angiogenesis by preventing nuclear localization of HIF-1α. Although it has been shown that TSGA10 messenger RNA (mRNA) is mainly expressed in testis and some tumors, the transcription pattern and regulatory mechanisms of this gene remain largely unknown. Here, we report that human TSGA10 comprises at least 22 exons and generates four different transcript variants. It was identified that using two distinct promoters and splicing of exons 4 and 7 produced these transcript variants, which have the same coding sequence, but the sequence of 5'untanslated region (5'UTR) is different between them. This is significant because conserved regulatory RNA elements like upstream open reading frame (uORF) and putative internal ribosome entry site (IRES) were found in this region which have different combinations in each transcript variant and it may influence translational efficiency of them in normal or unusual environmental conditions like hypoxia. To indicate the transcription pattern of TSGA10 in breast cancer, expression of identified transcript variants was analyzed in 62 breast cancer samples. We found that TSGA10 tends to express variants with shorter 5'UTR and fewer uORF elements in breast cancer tissues. Our study demonstrates for the first time the expression of different TSGA10 transcript variants in testis and breast cancer tissues and provides a first clue to a role of TSGA10 5'UTR in regulation of translation in unusual environmental conditions like hypoxia. © 2017

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Style	Citing Format
MLA	Salehipour P, et al.. "Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer." Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, vol. 1860, no. 9, 2017, pp. 973-982.
APA	Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH (2017). Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1860(9), 973-982.
Chicago	Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH. "Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer." Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 1860, no. 9 (2017): 973-982.
Harvard	Salehipour P et al. (2017) 'Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer', Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1860(9), pp. 973-982.
Vancouver	Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH. Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2017;1860(9):973-982.
BibTex	@article{ author = {Salehipour P and Nematzadeh M and Mobasheri MB and Afsharpad M and Mansouri K and Modarressi MH}, title = {Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer}, journal = {Biochimica et Biophysica Acta - Gene Regulatory Mechanisms}, volume = {1860}, number = {9}, pages = {973-982}, year = {2017} }
RIS	TY - JOUR AU - Salehipour P AU - Nematzadeh M AU - Mobasheri MB AU - Afsharpad M AU - Mansouri K AU - Modarressi MH TI - Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms VL - 1860 IS - 9 SP - 973 EP - 982 PY - 2017 ER -

Style

Citing Format

MLA

Salehipour P, et al.. "Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer." Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, vol. 1860, no. 9, 2017, pp. 973-982.

APA

Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH (2017). Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1860(9), 973-982.

Chicago

Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH. "Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer." Biochimica et Biophysica Acta - Gene Regulatory Mechanisms 1860, no. 9 (2017): 973-982.

Harvard

Salehipour P et al. (2017) 'Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer', Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1860(9), pp. 973-982.

Vancouver

Salehipour P, Nematzadeh M, Mobasheri MB, Afsharpad M, Mansouri K, Modarressi MH. Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms. 2017;1860(9):973-982.

BibTex

@article{ author = {Salehipour P and Nematzadeh M and Mobasheri MB and Afsharpad M and Mansouri K and Modarressi MH}, title = {Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer}, journal = {Biochimica et Biophysica Acta - Gene Regulatory Mechanisms}, volume = {1860}, number = {9}, pages = {973-982}, year = {2017} }

RIS

TY - JOUR
AU - Salehipour P
AU - Nematzadeh M
AU - Mobasheri MB
AU - Afsharpad M
AU - Mansouri K
AU - Modarressi MH
TI - Identification of New Tsga10 Transcript Variants in Human Testis With Conserved Regulatory Rna Elements in 5'Untranslated Region and Distinct Expression in Breast Cancer
JO - Biochimica et Biophysica Acta - Gene Regulatory Mechanisms
VL - 1860
IS - 9
SP - 973
EP - 982
PY - 2017
ER -

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