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Down-Regulation of Tsga10, Aurkc, Oip5 and Akap4 Genes by Lactobacillus Rhamnosus Gg and Lactobacillus Crispatus Sj-3C-Us Supernatants in Hela Cell Line; [Supernatanty Lactobacillus Rhamnosus Gg a Lactobacillus Crispatus Sj-3C-Us Snizuji Expresi Genu Tsga10, Aurkc, Oip5 a Akap4 V Hela Bunkach] Publisher Pubmed



Nouri Z1 ; Neyazi N1 ; Modarressi MH2 ; Karami F3 ; Abedindo A4 ; Taherianesfahani Z4 ; Ghafourifard S4 ; Motevaseli E5
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Authors Affiliations
  1. 1. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Medical Genetics, Science and Research Branch, Islamic Azad University, Tehran, Iran
  4. 4. Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Klinicka Onkologie Published:2018


Abstract

Background: Cancer testis antigens (CTAs) are considered cancer biomarkers due to their highly specific expression pattern in human malignancies and near absence from normal somatic tissues. Their specific expression has made them potential targets for early diagnosis, assessment of patients’ prognosis and treatment of cancer in recent years. Lactobacilli are a group of probiotics with anti-cancer, immunomodulatory and other beneficial features. These bacteria have been shown to alter expression of several cancer-related genes. Aim: We investigated the effect of Lactobacillus rhamnosus GG supernatant (LRS) and Lactobacillus crispatus SJ-3C--US supernatant (LCS) on expression of four CTAs (TSGA10, AURKC, OIP5 and AKAP4) in HeLa cell line after synchronization using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay and quantitative real-time polymerase chain reaction. Results: LRS and LCS inhibited HeLa cell growth after 24 h as demonstrated by MTT assay. Expressions of all CTAs were down-regulated after treatment with both supernatants. Conclusion: This study showed the role of Lactobacilli in down-regulation of CTAs genes. Such expression change might be involved in the anticancer effects of these Lactobacilli. The underlying mechanisms of these observations are not clear but epigenetic modulatory mechanisms may participate in this process. Future studies are needed to assess functional roles of Lactobacilli in modulation of other cancer-related genes. © 2019, Czech Medical Association J.E. Purkyne. All rights reserved.
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