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Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells Publisher Pubmed



Karami F1 ; Mohammadiyeganeh S2, 3 ; Abedi N1 ; Koochaki A3 ; Kia V4 ; Paryan M5
Authors
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Authors Affiliations
  1. 1. Tehran Medical Branch, Islamic Azad University, khaghani st, shariati Ave, PO Box 19395/1495, Tehran, Iran
  2. 2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Shahid Chamran Freeway, Velenjak, Tehran, Iran
  3. 3. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Shahid Chamran Freeway, Velenjak, Tehran, Iran
  4. 4. Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Azadi Sq., Jomhoori Slami Blv., Zanjan, Iran
  5. 5. Department of Research and Development, Production and Research Complex Pasteur Institute of Iran, 25th km of Tehran-karaj highway, PO Box 3159915111, Tehran, Iran

Source: Chemical Biology and Drug Design Published:2016


Abstract

Breast cancer is one of the most prevalent malignancies among women worldwide. Triple negative breast cancer (TNBC) is a type of breast cancer in which estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 (HER-2) are not expressed. There is no targeted therapy for this type of cancer, and available therapies have poor therapeutic effects. Performing a preliminary research, we selected cyclin D1 (CCND1) gene of Wnt signaling pathway which is a target of miRNAs, a promising set of biomolecules in diagnosis and treatment of breast cancer. In this study using bioinformatic analyses, miR-17 was selected as it targets the 3′UTR of CCND1 gene with the highest score. Luciferase assay results also confirmed the bioinformatic prediction. Decreased expression of miR-17 in MDA-MB-231 cell line was observed using qRT-PCR method. After lentiviral transduction of miR-17 to the target cells, gene expression analysis showed decreased expression of CCND1 gene. We found miR-17 as an attractive molecule that after intensive research can probably be used as a biomarker in TNBC. © 2015 John Wiley & Sons A/S.