Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells

Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells Publisher Pubmed

Karami F¹ ; Mohammadiyeganeh S^{2, 3} ; Abedi N¹ ; Koochaki A³ ; Kia V⁴ ; Paryan M⁵

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1. Tehran Medical Branch, Islamic Azad University, khaghani st, shariati Ave, PO Box 19395/1495, Tehran, Iran
2. Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Shahid Chamran Freeway, Velenjak, Tehran, Iran
3. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Taleghani Hospital, Shahid Chamran Freeway, Velenjak, Tehran, Iran
4. Department of Medical Biotechnology, Faculty of Medicine, Zanjan University of Medical Sciences, Azadi Sq., Jomhoori Slami Blv., Zanjan, Iran
5. Department of Research and Development, Production and Research Complex Pasteur Institute of Iran, 25th km of Tehran-karaj highway, PO Box 3159915111, Tehran, Iran

Source: Chemical Biology and Drug Design Published:2016

Abstract

Breast cancer is one of the most prevalent malignancies among women worldwide. Triple negative breast cancer (TNBC) is a type of breast cancer in which estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2 (HER-2) are not expressed. There is no targeted therapy for this type of cancer, and available therapies have poor therapeutic effects. Performing a preliminary research, we selected cyclin D1 (CCND1) gene of Wnt signaling pathway which is a target of miRNAs, a promising set of biomolecules in diagnosis and treatment of breast cancer. In this study using bioinformatic analyses, miR-17 was selected as it targets the 3′UTR of CCND1 gene with the highest score. Luciferase assay results also confirmed the bioinformatic prediction. Decreased expression of miR-17 in MDA-MB-231 cell line was observed using qRT-PCR method. After lentiviral transduction of miR-17 to the target cells, gene expression analysis showed decreased expression of CCND1 gene. We found miR-17 as an attractive molecule that after intensive research can probably be used as a biomarker in TNBC. © 2015 John Wiley & Sons A/S.

2. Is Mir-144 an Effective Inhibitor of Pten Mrna: A Controversy in Breast Cancer, In Vitro Cellular and Developmental Biology - Animal (2018)

3. Exosomal Mirnas From Highly Metastatic Cells Can Induce Metastasis in Non-Metastatic Cells, Life Sciences (2019)

Experts (# of related papers)

Alireza Biglari (2)

Style	Citing Format
MLA	Karami F, et al.. "Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells." Chemical Biology and Drug Design, vol. 87, no. 3, 2016, pp. 317-320.
APA	Karami F, Mohammadiyeganeh S, Abedi N, Koochaki A, Kia V, Paryan M (2016). Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells. Chemical Biology and Drug Design, 87(3), 317-320.
Chicago	Karami F, Mohammadiyeganeh S, Abedi N, Koochaki A, Kia V, Paryan M. "Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells." Chemical Biology and Drug Design 87, no. 3 (2016): 317-320.
Harvard	Karami F et al. (2016) 'Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells', Chemical Biology and Drug Design, 87(3), pp. 317-320.
Vancouver	Karami F, Mohammadiyeganeh S, Abedi N, Koochaki A, Kia V, Paryan M. Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells. Chemical Biology and Drug Design. 2016;87(3):317-320.
BibTex	@article{ author = {Karami F and Mohammadiyeganeh S and Abedi N and Koochaki A and Kia V and Paryan M}, title = {Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells}, journal = {Chemical Biology and Drug Design}, volume = {87}, number = {3}, pages = {317-320}, year = {2016} }
RIS	TY - JOUR AU - Karami F AU - Mohammadiyeganeh S AU - Abedi N AU - Koochaki A AU - Kia V AU - Paryan M TI - Bioinformatics Prediction and in Vitro Analysis Revealed That Mir-17 Targets Cyclin D1 Mrna in Triple Negative Breast Cancer Cells JO - Chemical Biology and Drug Design VL - 87 IS - 3 SP - 317 EP - 320 PY - 2016 ER -

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