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Helicobacter Pylori Strains From Duodenal Ulcer Patients Exhibit Mixed Baba/B Genotypes With Low Levels of Baba Adhesin and Lewis B Binding Publisher Pubmed



Saberi S1 ; Schmidt A2 ; Eybpoosh S3 ; Esmaili M1 ; Talebkhan Y1 ; Mohajerani N1 ; Oghalaie A1 ; Eshagh Hosseini M4 ; Mohagheghi MA5 ; Bugaytova J6 ; Boren T6 ; Mohammadi M1
Authors
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Authors Affiliations
  1. 1. HPGC Group, Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, 1316943551, Iran
  2. 2. Department of Medical Biosciences and Pathology, Umea University, Umea, 901 85, Sweden
  3. 3. Research Center for Modeling in Health, Institute for Future Studies in Health, Kerman University of Medical Sciences, Kerman, 7618747653, Iran
  4. 4. Department of Gastroenterology, Amiralam Hospital, Tehran University of Medical Sciences, Tehran, 1145765111, Iran
  5. 5. Cancer Research Center, Tehran University of Medical Sciences, Tehran, 141979733141, Iran
  6. 6. Department of Medical Biochemistry and Biophysics, Umea University, Umea, 901 87, Sweden

Source: Digestive Diseases and Sciences Published:2016


Abstract

Background: BabA is a Helicobacter pylori cell surface adhesin, which binds to the ABO/Leb histo-blood group antigens (Leb) and serves as a virulence factor. Methods: H. pylori single colonies were isolated from 156 [non-ulcer dyspepsia (NUD) = 97, duodenal ulcer (DU) = 34, gastric cancer (GC) = 25)] patients. babA and babB genes were evaluated by gene/locus-specific PCR. BabA protein expression and Leb binding activity were determined by immunoblotting and ELISA, respectively. Results: The combined categorization of H. pylori strains based on high, low or no levels of BabA expression and Leb binding, produced 4 groups: (I) BabA-high/Leb-high (36 %), (II) BabA-low/Leb-low (26 %), (III) BabA-neg/Leb-low (30 %) and (IV) BabA-neg/Leb-neg (8 %) strains. The majority (63 %) of the BabA-low/Leb-low strains exhibited mixed babA/B genotypes as compared to merely 18 % of the BabA-high/Leb-high, 15 % of the BabA-neg/Leb-neg and 11 % of the BabA-neg/Leb-low (P = 0.0001) strains. In contrast to NUD strains, the great majority (70 %) of DU strains were BabA-low/Leb-low (11 %, P = 0.0001), which compared to NUD strains, enhanced the risk of DU by 18.8-fold. In parallel, infection with babA/B mixed genotype strains amplified the risk of DU by 3.6-fold (vs. babA-positive: P = 0.01) to 6.9-fold (vs. babA-negative: P = 0.007). Conclusions: Here, we show higher prevalence of mixed babA/B genotypes among BabA-low/Leb-low clinical strains. Recombination of babA and babB genes across their loci may yield lower BabA expression and lower Leb binding activity. We conclude that H. pylori strains with lower Leb binding activity are better adapted for colonization of the gastric metaplastic patches in the duodenum and enhance the risk of duodenal ulcers. © 2016, Springer Science+Business Media New York.
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