Investigation of Association Between Icea, Baba2, and Oipa Genotypes of Helicobacter Pylori and Il-8-251 T>A Polymorphism With Clinical Outcomes in Helicobacter Pylori-Infected Iranian Patients Publisher



Akbari S¹ ; Rezaeian T¹ ; Mohammadzadeh R¹ ; Meshkat Z² ; Namdar AB³ ; Aryan E² ; Youssefi M² ; Pishdadian A⁴ ; Ahmadi A⁵ ; Farsiani H² Show Affiliations
Source: Gene Reports Published:2021

Abstract

More than half of the world's population is infected with Helicobacter pylori. Since the severity of clinical outcomes depends on pathogens, hosts, and environmental factors, we aimed to investigate the association between iceA, babA2, and oipA genotypes of H. pylori isolates and hosts IL-8-251 T>A polymorphism with clinical outcomes. PCR was performed on 193 cultured positive samples to identify genotypes of iceA, babA2, and oipA. PCR-RFLP was performed on blood samples of 144 hosts to determine IL-8-251 T>A polymorphism. Among patients, 133 (68.91%) had chronic gastritis, 44 (22.79%) had peptic ulcer disease (PUD), and 16 (8.2%) had gastric cancer (GC). All bacterial isolates were positive for the iceA, but the iceA1 allele was more frequent and it was most detected in PUD. The frequency of the oipA in different clinical outcomes was extremely high, and 100% GC-derived isolates were oipA gene-positive. However, there was no significant relationship between the iceA and oipA genotypes with clinical outcomes. Statistical analysis showed a significant relationship (p < 0.03) between the babA2 and clinical outcomes. The presence of the babA2 gene was more in patients with GC and PUD. The evaluation of IL-8-251 T>A polymorphism showed T/T (27.4%), A/A (22.9%), and A/T (49.3%) genotypes, but we could not detect a significant relationship between these genotypes and clinical outcomes. The prevalence of A/A and A/T genotypes was higher in subjects with PUD. The results of this study showed the association between babA2 genotype and serious clinical outcomes like PUD and GC. © 2021 Elsevier Inc.