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Transcriptomic and Proteomic Approaches Reveal Biological Basis of Intraoperative Radiotherapy-Treated Tumor Bed Modification in Breast Cancer Patients: A Pilot Study Publisher Pubmed



Shahani M1 ; Shakeri J1 ; Akbari ME1 ; Arefnezhad B2 ; Tafti A1, 3 ; Zali H4 ; Nafisi N5 ; Hashemi M6 ; Rezaeitavirani M7 ; Mohammadpour S1 ; Salami SAR8 ; Mirzai HR9 ; Samsami M1 ; Ezabady SHJ6 Show All Authors
Authors
  1. Shahani M1
  2. Shakeri J1
  3. Akbari ME1
  4. Arefnezhad B2
  5. Tafti A1, 3
  6. Zali H4
  7. Nafisi N5
  8. Hashemi M6
  9. Rezaeitavirani M7
  10. Mohammadpour S1
  11. Salami SAR8
  12. Mirzai HR9
  13. Samsami M1
  14. Ezabady SHJ6
  15. Akbari A1
Show Affiliations
Authors Affiliations
  1. 1. Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. OMICS Research Group (Media Teb Gene), Tehran, Iran
  3. 3. Departeman of Medical Biotechnology, Faculty of Medicine, Arak University of Medical Science, Arak, Iran
  4. 4. School of Advanced Technology in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  5. 5. Breast Department, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Genetics, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
  7. 7. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  8. 8. Department of Biotechnology, University of Tehran, Iran
  9. 9. Cancer Research Center, Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Proteomics Published:2020


Abstract

Aim: Intraoperative electron Radiotherapy, herein referred to, as IOeRT is a novel approach in breast cancer (BC) treatment. This study designed to investigate short-term molecular effects of 12Gy as Boost versus 21Gy as Radical dose of IOeRT using high throughput approaches. Materials and methods: Six BC patients as a pilot study were treated with IOeRT following two separate strategies, including Boost and Radical doses. Approximately 100 mg of tumor bed tissue retrieved from each patient (before IOeRT,immediately, 24 h post-treatment). mRNA sequencing also Isobaric tag for relative and absolute quantitation (iTRAQ) were performed to study the transcriptome and proteome profile of IOeRT-treated tumor bed. Results: Using NGS, ~6 Giga base (GB) clean data per individual samples were generated. Moreover, by iTRAQ for proteome quantification, in total, 1,045,410 spectrums were generated, likewise 5860 proteins were identified (FDR <0.01). Conclusion: Functional annotation and gene ontology (GO) indicated that significant enrichment in molecular pathways on BC treatment is somehow single high dose-independent. This means that, key molecular pathways in radiotherapy (RT) are equally enriched by both Boost and Radical doses. Generally, by modification of the Radical dose, with the same effectiveness, it is possible to reduce single high dose irradiation in BC. © 2019