Investigating the Efficacy of Dapsone in Treating Sepsis Induced by Cecal Ligation and Puncture Surgery in Male Mice Publisher Pubmed



Sayyad MS¹ ; Dehpour A¹ ; Poopak A¹ ; Azami A² ; Shafaroodi H¹ Show Affiliations
Source: Naunyn-Schmiedeberg's Archives of Pharmacology Published:2024

Abstract

Sepsis is a life-threatening condition caused by the body’s response to an infection. Dapsone is a sulfone with antibiotic properties, and experimental evidence suggests it has significant anti-inflammatory and anti-oxidative stress effects. The objective of this study was to investigate the efficacy of dapsone in mice after CLP (cecal ligation and puncture) surgery, which is a model for inducing sepsis. The study divided animals into five groups: CLP, sham, and three groups receiving different doses of dapsone (0.5, 1, 2 mg/kg). Sepsis was induced through CLP surgery, followed by dapsone administration. In each group, half of the mice were used to evaluate levels of various markers and pathological changes at 24 h post-CLP, while the other half was used to record the mortality rates within 96 h. The results showed that single-dose administration of dapsone at (0.5, 1, 2 mg/kg) after CLP surgery improved survival compared to the CLP group. Dapsone was also associated with a significant reduction in pro-inflammatory cytokines TNF-α, IL-1β, IL-6, NO, and MPO, as well as lactate and creatinine serum levels. However, dapsone did not have a significant effect on urea serum levels. In conclusion, the data suggest that dapsone treatment leads to increased survival in septic mice after CLP, and due to its ability to reduce TNF-α, IL-1β, IL-6, MPO, and lactate levels, it has anti-inflammatory effects in sepsis. The sepsis treatment with dapsone in mice protects against inflammation and oxidative stress. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.