Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
The Role of Mpl and Imiquimod Adjuvants in Enhancement of Immune Response and Protection in Balb/C Mice Immunized With Soluble Leishmania Antigen (Sla) Encapsulated in Nanoliposome Publisher Pubmed



Emami T1, 2 ; Rezayat SM1 ; Khamesipour A3 ; Madani R2 ; Habibi G4 ; Hojatizade M5 ; Jaafari MR6, 7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Proteomics and Biochemistry, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization(AREEO), Karaj, Iran
  3. 3. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Parasite Vaccine Research and Production, Razi Vaccine and Serum Research Institute, Agricultural Research Education and Extension Organization(AREEO), Karaj, Iran
  5. 5. Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran
  6. 6. Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
  7. 7. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2018


Abstract

Adjuvants play an essential role in the induction of immunity against leishmaniasis. In this study, monophosphoryl lipid A (MPL) and imiquimod (IMQ) were used as TLR ligands adjuvants to enhance immunogenicity and rate of protection against leishmaniasis. Nanoliposomes containing soluble Leishmania antigens (SLA) and adjuvants were consisted of DSPC, DSPG and Chol prepared by using lipid film method followed by bath sonication. The size of nanoliposomes was around 95 nm and their zeta potential was negative. BALB/c mice were immunized by liposomal formulations of lip/SLA, lip/MPL/SLA, lip/IMQ/SLA, lip/MPL/IMQ/SLA, lip/SLA + lip/IMQ, lip/SLA + lip/MPL, lip/SLA + lip/MPL/IMQ and five controls of SLA, lip/MPL, lip/IMQ, lip/MPL/IMQ and buffer by subcutaneously (SC) injections, three times in 2 weeks intervals. The synergic effect of two adjuvants when they are used in one formulation showed significantly (p <.001) smaller footpad swelling and the lowest parasite burden in lymph node and foot after the challenge. IgG2a in these groups showed the higher titre compared to control groups, which is compatible with the high IFN-γ production and lowest IL-4. Taken together the results indicated that co-delivery of MPL and IMQ adjuvants and antigen in nanoliposome carrier could be an appropriate delivery system to induce cellular immunity pathway against leishmaniasis. © 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
View other Related Docs
1. Development of Nano-Carriers for Leishmania Vaccine Delivery, Expert Opinion on Drug Delivery (2020)
2. Delivery Systems for Leishmania Vaccine Development, Expert Review of Vaccines (2016)
3. Evaluation of Immune Response Against Leishmaniasis in Balb/C Mice Immunized With Cationic Dotap/Dope/Chol Liposomes Containing Soluble Leishmania Major Antigens, Iranian Journal of Parasitology (2019)
4. Pegylation of Cationic Liposomes Encapsulating Soluble Leishmania Antigens Reduces the Adjuvant Efficacy of Liposomes in Murine Model, Parasite Immunology (2017)
5. The Role of Iscomatrix Bilayer Composition to Induce a Cell Mediated Immunity and Protection Against Leishmaniasis in Balb/C Mice, Iranian Journal of Basic Medical Sciences (2016)
6. The Role of Lpd-Nanoparticles Containing Recombinant Major Surface Glycoprotein of Leishmania (Rgp63) in Protection Against Leishmaniasis in Murine Model, Immunopharmacology and Immunotoxicology (2018)
7. A Phospholipase-A Activity in Soluble Leishmania Antigens Causes Instability of Liposomes, Current Drug Delivery (2020)
8. Cationic Immune Stimulating Complexes Containing Soluble Leishmania Antigens: Preparation, Characterization and in Vivo Immune Response Evaluation, Iranian Journal of Immunology (2015)
Experts (# of related papers)
View all Related Experts
Mahdi Mohebali (6)
Seyed Ebrahim Eskandari (6)
Other Related Docs
9. Evolution of Antigen-Specific Immune Responses in Cutaneous Leishmaniasis Patients, Parasite Immunology (2021)
10. Coadminstration of L. Major Amastigote Class I Nuclease (Rlmacin) With Lpd Nanoparticles Delays the Progression of Skin Lesion and the L. Major Dissemination to the Spleen in Balb/C Mice-Based Experimental Setting, Acta Tropica (2016)
11. Cationic Liposomes Formulated With a Novel Whole Leishmania Lysate (Wll) As a Vaccine for Leishmaniasis in Murine Model, Immunobiology (2018)
12. Multi-Antigen Vaccination With Lpd Nanoparticles Containing Rgp63 and Rlmac1n Proteins Induced Effective Immune Response Against Leishmaniasis in Animal Model, Journal of Drug Delivery Science and Technology (2021)
13. Combinatorial Delivery of Antigen and Tlr Agonists Via Plga Nanoparticles Modulates Leishmania Major-Infected-Macrophages Activation, Biomedicine and Pharmacotherapy (2021)
14. Double-Blind Randomized Efficacy Field Trial of Alum Precipitated Autoclaved Leishmania Major (Alum-Alm) Vaccine Mixed With Bcg Plus Imiquimod Vs. Placebo Control Group, Iranian Journal of Parasitology (2015)
15. Immunization Against Cutaneous Leishmaniasis by Alginate Microspheres Loaded With Autoclaved Leishmania Major (Alm) and Quillaja Saponins, Iranian Journal of Pharmaceutical Research (2016)
16. Comparison of Cytokine Profile of Ifn-Γ, Il-5 and Il-10 in Cutaneous Leishmaniasis Using Pbmc Vs. Whole Blood, Iranian Journal of Microbiology (2019)
17. An Overview of Leishmanization Experience: A Successful Control Measure and a Tool to Evaluate Candidate Vaccines, Acta Tropica (2019)
18. Effects of Combined Therapy With Silymarin and Glucantime on Leishmaniasis Induced by Leishmania Major in Balb/C Mice, Drug Research (2015)
19. Sphingomyelin Liposome Bearing Whole Leishmania Lysate Antigens Induce Strong Th2 Immune Response in Balb/C Mice, Iranian Journal of Basic Medical Sciences (2021)
20. Crispr/Cas9-Mediated Deletion of a Kinetoplast-Associated Gene Attenuates Virulence in Leishmania Major Parasites, Medical Microbiology and Immunology (2025)
21. Potential Biomarkers of Immune Protection in Human Leishmaniasis, Medical Microbiology and Immunology (2021)
22. Development of a New Live Attenuated Leishmania Major P27 Gene Knockout: Safety and Immunogenicity Evaluation in Balb/C Mice, Cellular Immunology (2018)
23. Liposomes: Structure, Biomedical Applications, and Stability Parameters With Emphasis on Cholesterol, Frontiers in Bioengineering and Biotechnology (2021)
24. Immune Response in Cutaneous Leishmaniasis Patients With Healing Vs. Non-Healing Lesions, Iranian Journal of Microbiology (2020)
25. Niosomal Formulation of Amphotericin B Alone and in Combination With Glucantime: In Vitro and in Vivo Leishmanicidal Effects, Biomedicine and Pharmacotherapy (2019)
26. Immunotherapeutic Effects of Glycyrrhiza Glabra and Glycyrrhizic Acid on Leishmania Major-Infection Balb/C Mice, Parasite Immunology (2022)
27. Safety Evaluation of Topical Application of Nano-Liposomal Form of Amphotericin B (Sinaampholeish) on Healthy Volunteers: Phase I Clinical Trial, Iranian Journal of Parasitology (2019)
28. Immunogenicity of Different Types of Adjuvants and Nano-Adjuvants in Veterinary Vaccines: A Comprehensive Review, Vaccines (2023)
29. Combination of Topical Liposomal Amphotericin B and Glucantime in Comparison With Glucantime Alone for the Treatment of Anthroponotic Cutaneous Leishmaniasis (Acl) Caused by Leishmania Tropica: Study Protocol for a Randomized, Controlled Trial, Iranian Journal of Microbiology (2021)
30. Anti Leishmania Activity of Lucilia Sericata and Calliphora Vicina Maggots in Laboratory Models, Experimental Parasitology (2016)
31. Rodenticide Comparative Effect of Klerat® and Zinc Phosphide for Controlling Zoonotic Cutaneous Leishmaniasis in Central Iran, Iranian Journal of Parasitology (2016)
32. Engineering and Design of Promising T-Cell-Based Multi-Epitope Vaccine Candidates Against Leishmaniasis, Scientific Reports (2023)
33. Emerging of Cutaneous Leishmaniais Due to Leishmania Major in a New Focus in Esfahan Province, Central Iran, Journal of Arthropod-Borne Diseases (2020)
34. Leishmania Major P27 Gene Knockout As a Novel Live Attenuated Vaccine Candidate: Protective Immunity and Efficacy Evaluation Against Cutaneous and Visceral Leishmaniasis in Balb/C Mice, Vaccine (2019)
35. The Leishmanicidal Effect of Lucilia Sericata Larval Saliva and Hemolymph on in Vitro Leishmania Tropica, Parasites and Vectors (2021)
36. Nanoliposomes Carrying Her2/Neu-Derived Peptide Ae36 With Cpg-Odn Exhibit Therapeutic and Prophylactic Activities in a Mice Tubo Model of Breast Cancer, Immunology Letters (2017)
37. Correlation Between Clinical Responses With the Drug-Susceptibility of Parasites in Iranian Cutaneous Leishmaniasis Caused by Leishmania Major, Tropical Biomedicine (2017)
38. Efficacy of Intra-Lesional Injections of Meglumine Antimoniate Once a Week Vs. Twice a Week in the Treatment of Cutaneous Leishmaniasis Caused by L. Tropica in Iran: A Randomized Controlled Clinical Trial, PLoS Neglected Tropical Diseases (2022)
39. Safety Evaluation of Nano-Liposomal Formulation of Amphotericin B (Sina Ampholeish) in Animal Model As a Candidate for Treatment of Cutaneous Leishmaniasis, Journal of Arthropod-Borne Diseases (2018)
40. P435 Her2/Neu-Derived Peptide Conjugated to Liposomes Containing Dope As an Effective Prophylactic Vaccine Formulation for Breast Cancer, Artificial Cells# Nanomedicine and Biotechnology (2019)
41. Dna Plasmid Coding for Phlebotomus Sergenti Salivary Protein Pssp9, a Member of the Sp15 Family of Proteins, Protects Against Leishmania Tropica, PLoS Neglected Tropical Diseases (2019)
42. Evaluation of G2 Citric Acid-Based Dendrimer As an Adjuvant in Veterinary Rabies Vaccine, Viral Immunology (2018)
43. The Therapeutic Effect of Larval Saliva and Hemolymph of Lucilia Sericata on the Treatment of Leishmania Major Lesion in Balb/C Mice946, Parasites and Vectors (2023)
44. Antileishmanial Activity of Urtica Dioica Extract Against Zoonotic Cutaneous Leishmaniasis, PLoS Neglected Tropical Diseases (2020)
45. Naloxone Effects Against Cutaneous Leishmaniasis Caused by Leishmania Major Strain Mrho/Ir/75/Er in the Balb/C Mice, Infectious Disorders - Drug Targets (2023)
46. Development of Topical Liposomes Containing Miltefosine for the Treatment of Leishmania Major Infection in Susceptible Balb/C Mice, Acta Tropica (2019)
47. Demographic and Clinical Characteristics of Children With Cutaneous Leishmaniasis in Isfahan Province, Iran, During 2011-2015, Journal of Isfahan Medical School (2017)
48. Sambucus Ebulus Extract Stimulates Cellular Responses in Cutaneous Leishmaniasis, Parasite Immunology (2019)
49. Evaluation of Cellular Immune Responses in Dogs Immunized With Alum-Precipitated Autoclaved Leishmania Major Along With Bcg and Imiquimod, Iranian Journal of Parasitology (2021)